Macromolecular Composition of the Liver Cell Formal Report Aims The aim of the experiment was to test for the presence of DNA‚ RNA‚ protein and glycogen in the cytoplasm and the nucleus of bovine liver cells. From the findings of the results the distribution of these macromolecules can be shown within the liver cell. This was carried out by undertaking qualitative experiments‚ where the observation of a colour change was noted and a quantitative experiment‚ where numerical data was recorded from
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Abstract The experiment‚ entitled Extraction and Characterization of Proteins‚ aims to isolate casein from milk and albumin from egg; to explain the methods employed for protein extraction; to apply spectrophotometric methods in characterizing and quantifying extracted casein and albumin. The experiment was divided into 2 parts; the extraction of Albumin from egg and the determination of protein concentration via the Warburg-Christian method and Bradford Assay method. In the first part‚ egg
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reducing sugars and this would be carried out by using Benedict’s test. To find out which substances had starch present using iodine To find out which substances were lipids using the emulsion test To test for proteins in the substances using the biuret test Context of the experiments: The context of this experiment was that we were interested in finding out what biological compounds we were dealing with as we had no idea what each substance was and all samples looked the same therefore we had
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How the structure of proteins are related to their functions Rachel Morris The basic building blocks of proteins are amino acids‚ the biuret reaction tests for protein. A solution of sodium hydroxide is added to a sample then a few drops of copper sulphate solution‚ if positive – the solution will turn mauve. There are 20 different amino acids and they can be joined in any order. Therefore there can be many different functions. A protein consists of one or more polypeptide chains (a polypeptide
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Protein Hydrolysis and Characterization Group 7 Niez‚ Robert Francis‚ *Orbin‚ Alfonso Ricardo* Parro‚ Athena Emmanuelle Peralta‚ Christian Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines • Abstract Hydrolyzed Protein is protein that has been hydrolyzed or broken down into its component amino acids. While there are many means of achieving this‚ two of the most common are prolonged boiling in a strong acid (acid-HVP) or strong base or using an enzyme such
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Exercise 1: Correct Use of the Scientific Method and an Experiment with Macromolecules I. Objectives In this lab‚ students will: a.) Learn how to utilize the Scientific Method to develop a testable hypothesis b.) Generate an effective experimental design from a hypothesis c.) Understand the chemical differences between different macromolecules and how these differences can be used to design tests for each molecule. d.) Identify macromolecules in solutions using chemical analyses. e.) Practice
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and attention will be paid to the correct use of the significant figures. The experiment approach that will be used will tell the mass and volume of the metal and liquid determined by measuring these two quantities with a graduated cylinder and biuret. Procedure: a) The density of metals First I obtained a quantity of unknown metal. Recording the unknown number. I used only one type of metal for this part of the experiment. The unknown metal that I was instructed to use was a chunk‚
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The quantitative and qualitative analytical techniques used‚ suggesting improvements for Future investigations D1 Titration: positive aspects of experiment in terms of accuracy -Checking to see if any air bubbles were present and if the burette was leaking before doing the titration. If I did not do this‚ it would alter the end point and hence the titre results. -Recording the volume reading before doing the titration. -Making sure I was
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Date 11/10/14 Partner if applicable Part I: Observations of formation of casein precipitate The milk began to curd. As the curd increases the milk appeared more clear at times. Mass of casein 1.65 g Part II: Test tube Observation of CuSO4 biuret Test Test Results (+ or -) Casein Solution turned violet + Egg white solution turned violet + Water solution turned blue - Sugar solution turned blue - Postlab Questions 1. Whas is the theoretical yield of casein based on the table of Milk Composition
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albumen is water. The remaining 10 % is made up of other substances such as reducing sugar. (a) Describe how you could safely test a sample of albumen for reducing sugar. [4] (b) A student tested some albumen for the presence of protein using Biuret reagent. The solution changed colour. It was a positive result. Describe this colour change. [1] © UCLES 2013 0610/61/M/J/13 www.maxpapers.com 3 (c) Fig. 1.2 shows an experiment to investigate the effect of acid on albumen. 3 drops
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