Veronica Guerrero Macromolecules of Life The macromolecules of life experiment involves the biology of life. The biology of life includes carbohydrates‚ lipids‚ proteins and which will not be included in this experiment nucleic acids. The introduction of this experiment will reference how to become familiar with lipids‚ carbohydrates‚ and proteins. It will also include the knowledge of how these macromolecules function in living organisms. This experiment will identify the macromolecules
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BSC 2010C.LAB TH 7-9:50pm 29 August 2013 Biology Lab Report Lab #1 –PROTEIN EXTRACTION LAB I. INTRODUCTION To begin the process of protein extraction and compare the results in a study‚ it is necessary to understand the importance of proteins‚ the process of extraction and how you are using the results to determine a rational conclusion. First understand proteins and the necessity of studying their impact. Proteins are essential molecules for biological functions and are the
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water (DW)‚ slurries of honey‚ egg white‚ and saltine crackers dropper of IKI solution (starch test) dropper bottle of Benedict’s reagent (glucose test) dropper bottles of 10% NaOH and 2% CuSO4 (Biuret Reagents – protein test) 95ºC water bath masking tape for labeling test tubes Procedure: Biuret test for a typical protein: Place a small piece of masking tape on 7 clean test tubes. Using a permanent marking pen‚ label the tubes 1-7. Add drops of solutions to each tube as follows: |Tube
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CAL Biology Practical 2 – Identification of Biochemicals in Pure Form Name :……………………………………………………………. MARK: Class :……………………………………………………………. PRACTICAL 2: IDENTIFICATION OF BIOCHEMICALS IN PURE FORM CAUTION: Any heating that has to be done in the following tests should be carried out in a water bath at the boiling point of water. Direct heating of test-tubes should not take place. Introduction Biochemical Tests Qualitative - present/ absent Semi-quantitative - present in >/<
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is the prediction because the belief is that Sample B is milk or cheese therefore containing lactose‚ fat‚ and protein. Methods Materials: 14 test tubes‚ 1 test tube rack‚ 1 paper towel‚ 5 ml Benedicts solution‚ .5 ml Iodine solution‚ 2 ml Biuret reagent‚ hot plate‚ samples 1-5‚ A‚ and B. Procedure: Obtain materials‚ Label the test tubes 1-5 ad A and B
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An international journal published by the Nigerian Society for Experimental Biology Printed in Nigeria Cofactor interactions in the activation of tissue non-specific alkaline phosphatase: Synergistic effects of Zn2+ and Mg2+ ions Femi J. OLORUNNIJI*‚ Adedoyin IGUNNU‚ Joseph O. ADEBAYO‚ Rotimi O. ARISE and Sylvia O. MALOMO Department of Biochemistry‚ University of Ilorin‚ P.M.B. 1515 Ilorin‚ Nigeria Received 19 March 2007 MS/No BKM/2007/028‚ © 2007 Nigerian Society for Experimental
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followed without any variations in materials and methods. The number for the unknown solution is‚ #128. Results The Results of Each Solution When Mixed with Each Type of Tests Test Tube Samples | Benedict Before After | Biuret | Iodine | #1. 1% glucose solution | light blue | red/brown | light blue | pale yellow | #2. 0.3% glucose - 1- phosphate | light blue | no colour change | light blue | pale yellow | #3. 1% maltose solution | light blue | red/brown | light blue
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These biological macromolecules are to be identified by the changes in colour through three different tests - Iodine Test for starch and glycogen‚ Benedict ’s Test for reducing sugars‚ and Biuret Test for Proteins. However‚ only two macromolecules are being identified in this experiment - carbohydrates and proteins. There are 12 solutions to be tested in this experiment. The Iodine test is used to indentify starch and glycogen in the given
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were mixed thoroughly. Test tube 1 was placed under a flame for observation. Colour change within the test tubes were recorded. Protein test 10 test tubes were used and lab-elled. 9 of the test tubes were filled with 5ml of food sample. 3 drops of Biuret reagent were added to each of the test tubes. The test tubes were gently shaken to mix the solution. Observation of colour change was
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in the 1940s. His 1951 paper describing the technique is the most-highly cited paper ever in the scientific literature‚ cited over 200‚000 times. The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method is best used with protein concentrations of 0.01–1.0 mg/mL and is based on the reaction of Cu+‚ produced by the oxidation of peptide bonds‚ with Folin–Ciocalteu reagent (a mixture
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