Biological Molecules Experiment-Specific Questions Experiment 1: Monosaccharide Test 1. Fill in the table below with the results from the monosaccharide test experiment‚ and your conclusions based on those results. Results Monosaccharide Test Solution Initial Color Color with Benedict’s Solution Color After Heating Monosaccharide? glucose solution clear blue orange yes water clear blue blue no sucrose solution clear blue
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is the prediction because the belief is that Sample B is milk or cheese therefore containing lactose‚ fat‚ and protein. Methods Materials: 14 test tubes‚ 1 test tube rack‚ 1 paper towel‚ 5 ml Benedicts solution‚ .5 ml Iodine solution‚ 2 ml Biuret reagent‚ hot plate‚ samples 1-5‚ A‚ and B. Procedure: Obtain materials‚ Label the test tubes 1-5 ad A and B
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followed without any variations in materials and methods. The number for the unknown solution is‚ #128. Results The Results of Each Solution When Mixed with Each Type of Tests Test Tube Samples | Benedict Before After | Biuret | Iodine | #1. 1% glucose solution | light blue | red/brown | light blue | pale yellow | #2. 0.3% glucose - 1- phosphate | light blue | no colour change | light blue | pale yellow | #3. 1% maltose solution | light blue | red/brown | light blue
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These biological macromolecules are to be identified by the changes in colour through three different tests - Iodine Test for starch and glycogen‚ Benedict ’s Test for reducing sugars‚ and Biuret Test for Proteins. However‚ only two macromolecules are being identified in this experiment - carbohydrates and proteins. There are 12 solutions to be tested in this experiment. The Iodine test is used to indentify starch and glycogen in the given
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were mixed thoroughly. Test tube 1 was placed under a flame for observation. Colour change within the test tubes were recorded. Protein test 10 test tubes were used and lab-elled. 9 of the test tubes were filled with 5ml of food sample. 3 drops of Biuret reagent were added to each of the test tubes. The test tubes were gently shaken to mix the solution. Observation of colour change was
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in the 1940s. His 1951 paper describing the technique is the most-highly cited paper ever in the scientific literature‚ cited over 200‚000 times. The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method is best used with protein concentrations of 0.01–1.0 mg/mL and is based on the reaction of Cu+‚ produced by the oxidation of peptide bonds‚ with Folin–Ciocalteu reagent (a mixture
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Danny Fish 10/9/11 Chemical Testing To identify An Unknown The hypothesis tested was that depending on the solution presented‚ which would test positive for one of the following‚ proteins‚ carbohydrates‚ or lipids through use of chemical testing. (Sudan IV‚ Benedicts’ Solution‚ Iodine‚ Biuret’s) . In order to gain more information for the hypothesis‚ one must know how to test for said macromolecule. Each of the above stated molecules has their own individual solution that will in turn identify
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Biological Molecules: Carbohydrates‚ Lipids‚ Proteins By the end of this lab‚ the student should be able to: Identify the functional groups for each of the biomolecules that react in the following biochemical tests: Benedict’s test‚ Iodine test‚ Brown Bag test‚ Sudan III/IV test‚ and the Biuret’s Test. Describe the mechanism of reaction for: Benedict’s test‚ Iodine test‚ Sudan III/IV test‚ and the Biuret’s Test. Interpret the results when presented with data for each of the biochemical
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What are the central ideas of Cells? • What are cells? – Cells are the smallest basic unit of living things. • What do they do? – Cells take in raw materials and making new substances. • Why do they do this? – In cells‚ organelles carry out activities to keep the organism alive. – E.g. synthesising proteins and fats‚ releasing energy from glucose. What are the central ideas of Movement of Substances? • What processes describe how substances move from one region to another? –
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Proteins Biuret Test Results. Figure 1 is showing both graphs of results from the spectrophotometer. Figure 1A is the results without the unknown plotted. By using the best fit line and equation the unknown substance was found to have .75 absorbance and 11.5 mg/mL
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