Osmosis is the diffusion of a solvent across a selectively permeable membrane that occurs in response to differences in solute concentrations (Allen and Harper 2014).Osmosis can fall under the category of passive transport which does not require energy. With osmosis being a type of diffusion it is viewed as molecules moving from a high concentration to a low concentration. To further explain if there is a low water concentration‚ high amounts of solutes will be present. Water will most likely move
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Macromolecules Gist of Experiment • Use different tests to check for the existence of macromolecules in various substances o Iodine test checks for starch and/or glycogen o Benedict’s test checks for reducing sugars o Biuret test checks for protein Notes on Underlying Theory Introduction • The most abundant elements in living material are: o Carbon o Hydrogen o Oxygen o Nitrogen o Sulfur o Phosphorus
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THE CHEMICAL CONSTITUENTS OF LIVING MATTER Relevant IB topic: T3 Time: 2 hours BACKGROUND The principal chemical constituents of living matter are: water‚ mineral salts‚ organic compounds such as carbohydrates‚ proteins‚ lipids‚ nucleic acids. In this exercise we shall concentrate on carbohydrates‚ lipids‚ proteins‚ and we shall estimate the concentration of Vitamin C‚ an organic compound‚ in a solution‚ by the
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solution called silver nitrate we could test whether or not salt was present in the urine. If the urine turned a cloudy white after adding the silver nitrate the urine had a high concentration of salt. To test for amino acid we used a chemical called biuret solution. If the urine turned the color purple it means that there was excess amino acid found in the urine. We then used a glucose test strip to test if the urine had glucose in it. If the strip turned a different color it meant that glucose was
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Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution [1]. There are several reagents that can be used to determine the concentration of protein in solution which are Bradford reagent‚ Biuret reagent‚ Lowry protein assay and many more. In this experiment‚ the Bradford Reagent is used to measure the protein concentration. Marion M. Bradford had developed the Bradford protein assay. The Bradford assay is a colorimetric assay based on the
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How the structure of proteins are related to their functions Rachel Morris The basic building blocks of proteins are amino acids‚ the biuret reaction tests for protein. A solution of sodium hydroxide is added to a sample then a few drops of copper sulphate solution‚ if positive – the solution will turn mauve. There are 20 different amino acids and they can be joined in any order. Therefore there can be many different functions. A protein consists of one or more polypeptide chains (a polypeptide
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ups Identifying food groups in unknown solutions Aim The aim of this experiment is to identify different food groups within several different unknown solutions. This will be carried out by placing the unknown solutions into separate test tubes and using different chemical indicators to see if any reactions occur. Each food group will act different when the chemical indicator is added‚ some will change colour while others will separate. Introduction Food is a vital source of energy that is
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edkfjeLkfkfkrfkrkg;regkldllllllllllllllllllllllllllllllllllllllllllllllllllllll- llllllllllllllllllllllllllllllllllllllllllllllllllllllllllll- llllllllllllllllllllllllllllllllllllllllllllllllllldfdkf Determine the presence of catalase enzyme activity using hydrogen peroxide. 1. Test for the presence of catalase in liver. A. Using a knife‚ cut a slice of liver that measures approximately 1 cm by 1 cm and place it on a slide. B. Using a micropipette‚ place a few drops of hydrogen peroxide (H2O2)
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unknown 1 (1 point) positive 5. unknown 2 (1 point) negative 6. unknown 3 (1 point) positive 7. unknown 4 (1 point) negative 1. List whether each of the following substances was positive or negative for protein‚ as indicated by the Biuret test. (7 points) 1. chicken soup (1 point) positive 2. water (1 point) negative 3. gelatin (1 point) positive 4. unknown 1 (1 point) positive 5. unknown 2 (1 point) negative 6. unknown 3 (1 point) negative
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3.1 Proteins Table 3.2 Biuret Test Tube Contents Final Color Conclusions 1 Distilled water Transparent‚ light blue‚ navy Possibly little protein with clear peptide or no protein at all 2 Albumin Dark Purple Proteins are present with purple peptides 3 Pepsin Purplish blue‚ darkish blue Proteins are present with purple or black colored peptides 4 Starch Light blue‚ really clear Possible little protein with clear peptide or no protein at all Our results are correct because water
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