Determination of presence of protein in a variety of food substances by the use of Biuret Reagent Biol. 1040 Section 1005 Introduction The human body consumes carbohydrates‚ lipids‚ and proteins each day by eating a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular
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groups can be used to identify molecules that are in that class. This lab is broken down into four different sections‚ the Benedict’s test for reducing sugars‚ the iodine test for the presence of starch‚ the Sudan III test for fatty acids‚ and the Biuret test for amino groups present in proteins. The last part of this lab takes an unknown substance and by the four tests‚ determine what the substance is. BENEDICT’S TEST Introduction: Monosaccharides and disaccharides can be detected because of
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tests that are commonly used to test for protein which are the Millon’s and Biuret’s test. A Millon’s test detects the amino acid tyrosine and if the proteins possess a certain substance‚ only then it will give out a positive result. However‚ the Biuret test is done to identify the presence of
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found?” The organic molecules being sugar‚ starch‚ and protein. The chemicals that are used in this experiment are Iodine‚ Biuret solution‚ and Benedict’s solution. Iodine is an element on the periodic table. It has an atomic number of 53. Iodine is used very often in natural history and is used to find starch‚ which will be needed in order to conduct this experiment. Biuret Solution is a mixture of copper sulfate and potassium hydroxide. It is used to find out how much protein if any is in something
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Name: ______________________________________ Date: ________________________ Student Exploration: Identifying Nutrients Vocabulary: carbohydrate‚ disaccharide‚ lipid‚ monosaccharide‚ polysaccharide‚ protein‚ starch Prior Knowledge Questions (Do these BEFORE using the Gizmo.) 1. What are the major types of nutrients you can get from food? Proteins‚ lipids‚ carbohydrates‚ minerals and vitamins 2. How are these nutrients used by your body? Nutrients are used by your body for
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Biruiet Test: Procedure Instead of the Biuret Reagent‚ the following may be used: * Fehling’s Solutions A and B * Sodium hydroxide and copper (II) sulphate solutions 1. Add 2 cm3 of the liquid food sample* to a clean‚ dry test tube 2. Add 2 cm3 of Biuret Reagent. Alternatively: * Use sodium hydroxide solution and copper sulphate solution instead. Add 1 cm3 of sodium hydroxide solution (40% or bench solution) and 1% copper (II) sulphate solution dropwise – drop by drop - to
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involves measuring the absorbance of several concentrations of the pure substance or the "standard" substance determine relationship between concentration and absorbance compared results from unknowns How to use Linear Regression for Generating a Standard Curve? © 2010 by M. Olaveson UTSC 2 BIO A01F-Fall 2010 - ASSIGNMENT # 2 - Preparing a Standard Curve using Excel 2007 Assignment # 2 Analysis of Data from Lab 2-Exercise 2 Table 2.6. Protein in Test Tubes prepared for
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By using the Sudan III test the presence of lipids can be found. Data Part 1 – Testing for Protein Description Observations 1 Egg white / albumin Purple‚ 8 drops 2 Pepsin solution Purple‚ 8 drops 3 Sugar No color change 4 Water No color change The Biuret reagent contains sodium hydroxide and copper
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total protein and albumin were calculated with the interpretation of a standard curve. To determine the concentration of total protein in human serum‚ a total protein assay was performed by using the Biuret reagent. Proteins react to this compound because it contains two amide bonds. When the Biuret reagents is introduced to a sample of protein‚ it forms a blue or violet complex. The concentration of albumin in human serum is determined by using the Bromocresol green (BCG) reagent. When albumin and
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Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free amino group and a free carboxylic acid
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