and the second session was 3.55). We then proceeded by adding 25 mL of 0.5 M of HCl. The pH values after this addition are 1.56 and 1.34. We filled a cleansed biuret with 0.5 M NaOH solution. The main process begins with us closely monitoring changes in pH as we added NaOH from the biuret at 0.5 mL increments. We refilled the biuret with NaOH when it neared empty. We halted the titration process when both the pH stabilized and we reached a pH level range of 12-13. All data collected was graphed
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When mixed with water and the tested substance‚ a positive result will occur in it being separated from the water. The objective of the Protein Test was to test for proteins present in a substance using the Biuret Solution. The substance should produce a violet color within 10 drops of Biuret. The objectives of the Spit Lab were to test for starch‚ a reducing sugar‚ and effect of amylase on a piece of bread that is positive for starch and negative for a reducing sugar. Iodine would test if starch
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Test for the Presence of Macromolecules Introduction In this experiment‚ we are trying to identify the presence of macromolecules in certain solutions. As in our daily lives‚ macromolecules are the essential nutrients that we have to take every day‚ therefore we would like to identify the types of macromolecules that contain in some of the food or solution in order to maintain a healthy life. There are four groups of macromolecules which are carbohydrates‚ lipids‚ proteins and
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were tested within the foods applesauce‚ peas‚ and turkey gravy. These biomolecules were identified using the solution indicators for the Biomolecules which are respectively designated for each biomolecule. Iodine for Starch‚ Benedicts for Sugar‚ Biurets for Protein‚ and a brown paper bag for Lipids. The biomolecules were observed when the food and indicators were combined‚ the solution then turned a color designating a specific biomolecule. Protein turns purple‚ Iodine turns black/blue‚ Sugar turns
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starch protein d. Biuret test - glucose tap water oil (lipid)) starch protein 7. All proteins contain carbon‚ hydrogen‚ oxygen and what other element? Nitrogen 8. When one glycerol molecule covalently bonds via dehydration synthesis with three fatty acid molecules the resulting macromolecule is called a __lipid_________. 9. What are the two general categories of carbohydrates? Starch and cellulose ID the test: Sudan IV‚ Benedict’s‚ Biuret‚ Starch 10. The
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only substance who turned shiny afterwards. All of the other ones were matte. Describe the positive test for protein. Explain how you know. The positive test for protein is adding the Biuret solution. I know this because the protein is the substance who had the most dramatic reaction with the Biuret Solution. While all of the other substances turned blue and yellow‚ the protein turned purple. What substances were present in the unknown solution? Explain how you know. The glucose
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Bradford Protein Assay Practical Report 1. Present your data (including raw data and calculated concentrations) for the protein standards in the form of a clear table. Give one example of how you calculated protein concentration. Do not forget a descriptive title and units (4marks) Title either too long or not descriptive or absent Your results are in duplicate & shouldn’t be referred to as ‘set1 & set2’ or ‘original’ and ‘duplicate’ Many of you think units of absorbance are nm but A has arbitrary
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molecules. These tests take advantage of the fact that different types of molecules have different functional groups and‚ therefor‚ different chemical properties. Thus‚ they will react to test reagents (also called indicators) differently. Materials: Biuret‚ Sudan III‚ Iodine‚ Benedict‚ water‚ hot plate‚ paper towels‚ pipette‚ ruler‚ goggles‚ and a timer. Method: Benedict’s test for reducing sugar: 1. Ruler was used to mark 1 cm and 2 cm marks on each tubes. 2. Negative control tube was filled with
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record their results. 7. Have students return to their desks but stay with their groups to complete the Analysis Questions in their journals. Alternate indicators: Iodine for lipids/oil (turns pink but transformation is temporary so watch carefully) Biuret Solution is easy to make. Start with 40% NaOH and
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Version 42-0085-00-01 Lab Report Assistant ________________ Name: Akil Kelly Exercise 1: Testing for Proteins Data Table 1: Biuret results. Substance Tested Predicted Results Biuret Color & Number of drops added 1: Egg white Contains Protein Purple color – 9 drops 2: Pepsin Contains Protein Purple color – 9 drops 3: Sugar Doesn’t contain protein Blue color – 9 drops 4: Distilled water Doesn’t
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