reduced copper in alkaline solutions. This is called a "Biuret" chromophore. The second is the reduction of Folin-Ciocalteu reagent (phosphomolybdate and phosphotungstate) by tyrosine and tryptophan residues. The reduced Folin-Ciocalteu reagent is blue and therefore is detectable with a spectrophotometer in the range of 750 nm. Using the Folin-Ciocalteu reagent to detect reduced copper makes the assay nearly 100 times more sensitive than the Biuret reaction alone. The BCA Assay: The BCA assay is
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RNA Analysis 1. The color result is light brown. 2. A positive Biuret test indicates the presence of proteins in the filtrate. Biuret is used to test for proteins. It is a light blue color and changes to purple in the presence of protein‚ a polymer made of amino acids. It will not change color when only amino acids are present. 3. What type of molecules present in the suspension is hydrolyzed by the mild acid treatment? 4. The products of hydrolysis are peptones‚ peptides‚ and amino acids
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ntroduction The purpose of this experiment was to use Iodine‚ Benedict and Biuret to test the reaction of the following 12 samples: 1% glucose‚ 0.3% glucose-1-phosphate‚ 1% maltose‚ honey‚ 1% sucrose‚ 1%lactose‚ 1% glycogen‚ 1% starch‚ protein‚ beer‚ distilled water and an unknown solution (test tube: 300). The iodine test for starch was to test how would starch reacted if we put iodine in it. The color of starch before the test was clear. The color of the iodine was brown. When you added iodine
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Title Biochemical Analysis of Blood Abstract Introduction After blood is collected from a patient it is analysed to check the fragments present and the fluid which will either be plasma or serum. After plasma is separated from blood it recollects the fibrinogen which in turn helps in clotting however serum is the portion of blood which remains after the fibrinogen is removed. Serum contains approximately 6-8% of the proteins which make up blood however plasma creates up to 55% of blood
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Lipids And Nucleic Acids Professor Anthony Depass Biology 1 9/25/14 Introduction: By completing lab exercise 6 : Biologically Important Molecules‚ we are able to perform tests such as the Benedicts Test for Reducing Sugars‚ Iodine Test‚ Biuret Test‚ Sudan IV Test‚ and lastly the Grease-Spot Test that detect the presence of biologically important carbohydrates‚ protein lipids and nucleic acids. Most known compounds in living organism are if fact carbohydrates‚ lipids‚ proteins‚ and nucleic
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this lab we will test four foods to see if they contain carbohydrates (sugar and/or starches)‚ proteins and lipids. We will use chemical reagents to test to determine the presence of macromolecules: Benidicts solution for sugar‚ Iodine for starch‚ Biurets reagent for protein‚ and Sudan III for lipids. Hypothesis (1 point) If we test buttermilk biscuits for starches‚ sugars‚ proteins and lipids then it will test positive for all. If we test potato chips for starches‚ sugars‚ proteins and lipids it
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Q 1. In this practical exercise you will be using biuret reagent. Why is biuret reagent used in this practical? The biuret reagent is used to assess the concentration of the protein because peptide bonds occur with the same frequency per amino acid in the peptide. The intensity of the colour‚ and therefore the absorption‚ is directly proportional to the protein concentration. Q 2. What is the experimental reason for constructing a standard curve? Multiple samples with known properties
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Analysis: Lab 1 Identifying Carbohydrates There were many ranges of color changes in the Benedicts test and Iodine stain. For the Benedicts test‚ this oxidation/reduction response changes the arrangement of the reducing sugar creating a colored precipitate. The change in color is correlated to the first concentration of the reducing sugar. For the iodine test‚ the three dimensional structure of various polysaccharides permits them to react with the iodine stain to produce a certain color. As far
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mellitus‚ a severe metabolic disorder due to defective carbohydrate utilization. Protein 1. Using a 10 ml graduated cylinder‚ add 1 ml urine to a clean glass vial. 2. Rinse the graduated cylinder and measure 2 ml biuret reagent. Note the pale blue color of the biuret reagent. 3. Add the biuret reagent to the urine
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Bio 6 (Mon. Lab) 5/6/13 The Effects of Pepsin VS. Trypsin in The Digestion of Protein Introduction As food is mechanically and chemically digested through our oral cavity then passes through our pharynx and down our esophagus‚ our food then enters the stomach. The stomach‚ being the main organ for storage‚ also helps with breaking down our food‚ but in order to accomplish that our pancreas helps by excreting an inactive digestive enzyme called pepsinogen. Pepsinogen is the inactive form
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