concentration of total protein in human serum‚ a total protein assay was performed by using the Biuret reagent. Proteins react to this compound because it contains two amide bonds. When the Biuret reagents is introduced to a sample of protein‚ it forms a blue or violet complex. The concentration of albumin in human serum is determined by using the Bromocresol green (BCG) reagent. When albumin and the BCG reagent are combined‚ the solution turns into a blue-green complex. Albumin and globulin are protein
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In this lab we employed various assays utilizing a biuret reagent‚ coomassie brilliant blue reagent‚ and ultraviolet light in order to determine the identity of six unknown solutions and the concentration of a bovine serum albumin sample. We were given three samples that lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used
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Biruiet Test: Procedure Instead of the Biuret Reagent‚ the following may be used: * Fehling’s Solutions A and B * Sodium hydroxide and copper (II) sulphate solutions 1. Add 2 cm3 of the liquid food sample* to a clean‚ dry test tube 2. Add 2 cm3 of Biuret Reagent. Alternatively: * Use sodium hydroxide solution and copper sulphate solution instead. Add 1 cm3 of sodium hydroxide solution (40% or bench solution) and 1% copper (II) sulphate solution dropwise – drop by drop - to
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Abstract The objective of this lab was to measure the amount of protein from a piece of beef liver . This was done by taking the liver‚ blending it and then using a centrifuge to separate the supernatant from the pellet. Once that was completed‚ ammonium sulfate was added to the supernatant‚ chilled and then spun for a second time. Next‚ 20 mL of water is added to the pellet‚ stirred and the volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic
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Version 42-0085-00-01 Lab Report Assistant ________________ Name: Akil Kelly Exercise 1: Testing for Proteins Data Table 1: Biuret results. Substance Tested Predicted Results Biuret Color & Number of drops added 1: Egg white Contains Protein Purple color – 9 drops 2: Pepsin Contains Protein Purple color – 9 drops 3: Sugar Doesn’t contain protein Blue color – 9 drops 4: Distilled water Doesn’t
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groups can be used to identify molecules that are in that class. This lab is broken down into four different sections‚ the Benedict’s test for reducing sugars‚ the iodine test for the presence of starch‚ the Sudan III test for fatty acids‚ and the Biuret test for amino groups present in proteins. The last part of this lab takes an unknown substance and by the four tests‚ determine what the substance is. BENEDICT’S TEST Introduction: Monosaccharides and disaccharides can be detected because of
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various types of biological molecules. These tests take advantage of the fact that different types of molecules have different functional groups and‚ therefor‚ different chemical properties. Thus‚ they will react to test reagents (also called indicators) differently. Materials: Biuret‚ Sudan III‚ Iodine‚ Benedict‚ water‚ hot plate‚ paper towels‚ pipette‚ ruler‚ goggles‚ and a timer. Method: Benedict’s test for reducing sugar: 1. Ruler was used to mark 1 cm and 2 cm marks on each tubes. 2. Negative
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oil Oats and water 20 ml gelatin and water solution 20 ml potato and water mixture 20 ml apple juice and water mixture 20 ml unknown substance #1 20 ml unknown substance #2 Paper towels 600 ml beaker Brown paper Biuret reagent Benedict’s solution Iodine solution Procedure: 1. Place test tubes in the test tube rack. Use the grease pencil to label each test tube based on the substance that will be placed in them. 2. To test for starches‚ fill each test tube
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Identification of Macromolecules Introduction The purpose of this lab is to test for the presence of macromolecules in various foods. Tests are performed to describe the basic structure of proteins‚ lipids‚ glycerides‚ and carbohydrates. We are to recognize the positives and negatives in these macromolecules. Also we are to identify the roles that proteins‚ lipids‚ minerals‚ vitamins‚ and carbohydrates play in the body’s construction and metabolism. Procedure / Results 5.1 Carbohydrate
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BIOLOGICALLY IMPORTANT MOLECULES Abstract Compounds containing the element carbon‚ called organic compounds‚ are the most important substances that make up living organisms. There are thousands and thousands of different organic compounds. To identify the major types of organic compounds in living organisms ‚ several biochemical test were used . Introduction Organic molecules are those primarily made up of carbon‚ hydrogen and oxygen. The common organic compounds of living organisms are
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