In the lab‚ three experiments were completed: the Iodine experiment‚ Benedict’s experiment and the Biuret experiment to test for starch and glycogen‚ reducing sugars and protein in the respective order. 12 solutions were given where 11 were known and the 12th was an unknown. The solutions that were supplied can be found in the results and observations section of this report. When completing such experiments‚ there are always controls used to make sure the procedure is followed and that no cross
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Title Biochemical Analysis of Blood Abstract Introduction After blood is collected from a patient it is analysed to check the fragments present and the fluid which will either be plasma or serum. After plasma is separated from blood it recollects the fibrinogen which in turn helps in clotting however serum is the portion of blood which remains after the fibrinogen is removed. Serum contains approximately 6-8% of the proteins which make up blood however plasma creates up to 55% of blood
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Macromolecular Composition of the Liver Cell Formal Report Aims The aim of the experiment was to test for the presence of DNA‚ RNA‚ protein and glycogen in the cytoplasm and the nucleus of bovine liver cells. From the findings of the results the distribution of these macromolecules can be shown within the liver cell. This was carried out by undertaking qualitative experiments‚ where the observation of a colour change was noted and a quantitative experiment‚ where numerical data was recorded from
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We can use Ninhydrin solution instead of biuret reagent. While using biuret reagent in performing this experiment does not require any heating up‚ using ninhydrin solution will need a process of heating up. After the process of heating up‚ violet-colored complex will develop in the presence of proteins‚ similar to biuret reagent‚ as ninhydrin solution reacts with amino acids which are present in the proteins. We can also repeat the experiment
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tests? The water was tested for each initial chemical test because it served as the controlled variable. Since water is pure oxygen and hydrogen unlike Glucose and Starch‚ one variable may be observed. 2. Biuret reagent will turn your skin brownish-purple. Explain why this occurs. Biuret reagent will turn your skin a brownish-purple because Iodine detects protein in a substance by changing the color to a brown or purple shade‚ and since your skin contains protein it will also change color. 3. When
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which can then be measured using colorimetric techniques. It is named for the biochemist Oliver H. Lowry who developed the reagent in the 1940s. His 1951 paper describing the technique is the most-highly cited paper ever in the scientific literature‚ cited over 200‚000 times. The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method is best used with protein concentrations of 0.01–1
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! EXERCISE 6! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! BIOLOGICALLY IMPORTANT MOLECULES! REPORT ! ! ! INTRODUCTION ! In this experiment‚ we will learn about biological important molecules by performing tests to detect the presence of carbohydrates‚ proteins‚ lipids‚ and nucleic acids. Also‚ we will use biochemical tests to identify the unknown compound that react in the following biochemical tests: Benedict’s test‚ Iodine test‚ Sudan IV test‚ Biuret’s
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includes to find the acidity and basicity of the fertilizer with the help of pHsolution. Morever I would also find out the solubility of the fertilizer in universal and organic solvent. To end the analysis I would furthermore test the presence of biuret which is toxic for plants and thus impairs plant growth. PRINCIPLE Qualitative analysis means to analyse cations and anions and other elements present in the given samples. The physical and chemical `properties will be observed for the different
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reducing sugars and this would be carried out by using Benedict’s test. To find out which substances had starch present using iodine To find out which substances were lipids using the emulsion test To test for proteins in the substances using the biuret test Context of the experiments: The context of this experiment was that we were interested in finding out what biological compounds we were dealing with as we had no idea what each substance was and all samples looked the same therefore we had
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Protein Hydrolysis and Characterization Group 7 Niez‚ Robert Francis‚ *Orbin‚ Alfonso Ricardo* Parro‚ Athena Emmanuelle Peralta‚ Christian Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines • Abstract Hydrolyzed Protein is protein that has been hydrolyzed or broken down into its component amino acids. While there are many means of achieving this‚ two of the most common are prolonged boiling in a strong acid (acid-HVP) or strong base or using an enzyme such
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