solution turns black-blue than starch is present (positive test). If the solution remains light yellow color‚ there is no starch present (negative test). We use the Benedict’s test for Simple Sugars to test for the presence of glucose. Benedict’s reagent is clear blue. A positive reaction in a Benedict’s test is the change of the clear light blue (negative test) solution to an orange solution in boiling water. This color change indicates the presence of glucose in a given solution. As we all know
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difference between organic and inorganic molecules. 6. Be familiar with the biochemical tests performed in lab-including the Millon test‚ Biuret test‚ Benedict’s test‚ Iodine test‚ Grease spot and Sudan Dye test. Know what each test is used for- protein (peptide bonds or tyrosine groups)‚ glucose‚ sucrose‚ starch or lipids. You should know the chemicals/reagents in each test and the results that would indicate a positive or negative for each biochemical. 7. Define and understand the concepts behind
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Carbohydrates‚ Proteins‚ lipids‚ and nucleic Acid Lab Exercise 6 Date: 9/17/12 Bio 102-11 Purpose the purpose of this experiment was to perform test to detect the presence of carbohydrates‚ proteins‚ lipids‚ and nucleic acids. Explain the importance of a positive and a negative control in biochemical test. Use biochemical test to identify an unknown compound. Background Most organic compounds in living organisms are carbohydrates‚ proteins‚ lipids‚ and nucleic acids they are called macromolecules
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the prediction because the belief is that Sample B is milk or cheese therefore containing lactose‚ fat‚ and protein. Methods Materials: 14 test tubes‚ 1 test tube rack‚ 1 paper towel‚ 5 ml Benedicts solution‚ .5 ml Iodine solution‚ 2 ml Biuret reagent‚ hot plate‚ samples 1-5‚ A‚ and B. Procedure: Obtain materials‚ Label the test tubes 1-5 ad A and B
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What are the central ideas of Cells? • What are cells? – Cells are the smallest basic unit of living things. • What do they do? – Cells take in raw materials and making new substances. • Why do they do this? – In cells‚ organelles carry out activities to keep the organism alive. – E.g. synthesising proteins and fats‚ releasing energy from glucose. What are the central ideas of Movement of Substances? • What processes describe how substances move from one region to another? –
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ntroduction The purpose of this experiment was to use Iodine‚ Benedict and Biuret to test the reaction of the following 12 samples: 1% glucose‚ 0.3% glucose-1-phosphate‚ 1% maltose‚ honey‚ 1% sucrose‚ 1%lactose‚ 1% glycogen‚ 1% starch‚ protein‚ beer‚ distilled water and an unknown solution (test tube: 300). The iodine test for starch was to test how would starch reacted if we put iodine in it. The color of starch before the test was clear. The color of the iodine was brown. When you added iodine
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The digestion of milk fats by pancreatic lipase and the digestion of proteins by pepsin and trypsin By Jenny Lam Abstract The purpose of this project was to determine how pancreatic lipase and bile salts digest fats to fatty acids and glycerol and how pepsin and tryspin digest to polypeptides. Litmus solution (pH indicator) was used for the fats to indicate the occurrence of the digestion of fats in experiment A. Egg whites was used as the substrate for the enzymes in experiment B.
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ntroduction In this experiment we created standards to be able to compare a mystery substance off of. We were tasked to investigate a murder that took place by analyzing a man’s last meal to find out where he had ate based on the content of his stomach. Before we could test the mystery substance we had to establish standards‚ so we could compare off of them. Based on the results of this lab‚ we believed that the victim last ate at Buffalo Wild Wings (Veliz). Methods and Materials We started this
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3.1 Proteins Table 3.2 Biuret Test Tube Contents Final Color Conclusions 1 Distilled water Transparent‚ light blue‚ navy Possibly little protein with clear peptide or no protein at all 2 Albumin Dark Purple Proteins are present with purple peptides 3 Pepsin Purplish blue‚ darkish blue Proteins are present with purple or black colored peptides 4 Starch Light blue‚ really clear Possible little protein with clear peptide or no protein at all Our results are correct because water
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essential nutrients‚ vitamins‚ and minerals play very significant functions in the different metabolic processes. In order for the nutrients to be known‚ several tests were performed to qualitatively analyze nutrients for the different food samples. Reagents were used as indicators to test common food substances for the presence of specific nutrients. Fruit juice‚ raw egg yolk‚ peanuts tested negative for mineral matter; margarine‚ raw egg yolk‚ peanuts tested positive for the presence of fat; fruit
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