It is a very good mechanism for transport since they are highly cohesive 5. Important reagents for chemical reactions ( such as hydrolysis and photosynthesis ) ------------------------------------------------- Carbohydrates Separated into 3 types which are monosaccharides‚ disaccharides and polysaccharides Monosaccharides * they
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involves measuring the absorbance of several concentrations of the pure substance or the "standard" substance determine relationship between concentration and absorbance compared results from unknowns How to use Linear Regression for Generating a Standard Curve? © 2010 by M. Olaveson UTSC 2 BIO A01F-Fall 2010 - ASSIGNMENT # 2 - Preparing a Standard Curve using Excel 2007 Assignment # 2 Analysis of Data from Lab 2-Exercise 2 Table 2.6. Protein in Test Tubes prepared for
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Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free amino group and a free carboxylic acid
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for Detecting Fats: The Grease Spot Test Perform the grease spot test‚ which will help to detect fats. Part C- Test for Detecting Protein In this part we perform the xanthoprotic‚ biuret‚ and ninhydrin test. A yellow color is positive for the xanthoprotic test‚ and tyrosine will give a positive result. For the biuret test‚ a purple color is a positive test. The ninhydrin test will give a positive result for glycine. Part D Testing Foods Perform the grease spot test to the substance after filtering
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Student Exploration: Identifying Nutrients Directions: In this online lab‚ you will be practicing identifying nutrients found in food. 1. Go to explorelearning.com 2. Login using the username/password your online teacher gave you at the beginning of the semester. If you forgot it‚ check your messages in blackboard. Most likely your username and password are your FirstnameLastname (no spaces). 3. Find the “Identifying Nutrients” gizmo. 4. Click on launch. Let’s get started.
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The use of positive and negative controls to determine the macromolecules present in an unknown solution Course # 6130‚ Wednesday‚ 7-10 PM‚ B2-151 Lab performed: September 21st‚ 2011 INTRODUCTION Every living thing is dependent on large complex molecules‚ known as macromolecules. The objective of this lab was to correctly identify which macromolecules the unknown solution was comprised of using various substances as experimental controls. There are four major types of biological macromolecules
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VARIATION 29 ECOLOGY 31 MARK SCHEMES 35 ORGANISMS IN THE ENVIRONMENT (Skill: Dr) Lab #: Date: Title: Classification of Animals – Insects Aim: To draw and label the external dorsal features of the cock roach of the genus Blaberus. Apparatus/Reagent/Material: Chloroform‚ hand lens‚ cock roach‚ pins‚ cardboard Method: 1. Collect a cockroach and put it in a bottle with cotton wool soaked in chloroform for 30mins. 2. Take the cockroach out and place it on the board with the dorsal side up‚ with
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and the second session was 3.55). We then proceeded by adding 25 mL of 0.5 M of HCl. The pH values after this addition are 1.56 and 1.34. We filled a cleansed biuret with 0.5 M NaOH solution. The main process begins with us closely monitoring changes in pH as we added NaOH from the biuret at 0.5 mL increments. We refilled the biuret with NaOH when it neared empty. We halted the titration process when both the pH stabilized and we reached a pH level range of 12-13. All data collected was graphed
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When mixed with water and the tested substance‚ a positive result will occur in it being separated from the water. The objective of the Protein Test was to test for proteins present in a substance using the Biuret Solution. The substance should produce a violet color within 10 drops of Biuret. The objectives of the Spit Lab were to test for starch‚ a reducing sugar‚ and effect of amylase on a piece of bread that is positive for starch and negative for a reducing sugar. Iodine would test if starch
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Test for the Presence of Macromolecules Introduction In this experiment‚ we are trying to identify the presence of macromolecules in certain solutions. As in our daily lives‚ macromolecules are the essential nutrients that we have to take every day‚ therefore we would like to identify the types of macromolecules that contain in some of the food or solution in order to maintain a healthy life. There are four groups of macromolecules which are carbohydrates‚ lipids‚ proteins and
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