observed. A pancreatin solution was first prepared from a hog pancreas and was completely neutralized using 0.5% Na2CO3. Afterwards‚ 3 mL each of the neutral pancreatin solution was placed in 7 test tubes and were further added with other specified reagents. The test tubes containing HCl were not effective in digesting the biomolecules since pancreatin is only active in moderately alkaline conditions‚ as observed in test tube 2. Data obtained from test tubes 3-7 were inconclusive and would most likely
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only substance who turned shiny afterwards. All of the other ones were matte. Describe the positive test for protein. Explain how you know. The positive test for protein is adding the Biuret solution. I know this because the protein is the substance who had the most dramatic reaction with the Biuret Solution. While all of the other substances turned blue and yellow‚ the protein turned purple. What substances were present in the unknown solution? Explain how you know. The glucose
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record their results. 7. Have students return to their desks but stay with their groups to complete the Analysis Questions in their journals. Alternate indicators: Iodine for lipids/oil (turns pink but transformation is temporary so watch carefully) Biuret Solution is easy to make. Start with 40% NaOH and
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Practical 2: Observation of mammalian kidney model and tissue slides. Analyzing kidney filtration using simple filtration system Introduction: Kidney is part of mammalian’s body endocrine system. Every mammals have a pair of kidneys that is located at the middle back of the body and symmetrically beside the spine and below the rib cage. A kidney approximately 0.5% of the organism body weight. Every kidney will receive huge amount of blood to enable them to perform important task. The base unit of
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Biology LabPaq / Published by: Hands-On Labs‚ Inc. sales@HOLscience.com / www.HOLscience.com / Toll Free 866.206.0773 A laboratory Manual of Small-Scale Experiments for the independent Study of general Biology 50-0053-BK-02 LabPaq® is a registered trademark of Hands-On Labs‚ Inc. (HOL). The LabPaq referenced in this manual is produced by Hands-On Labs‚ Inc. which holds and reserves all copyrights on experiences. The laboratory manual included with a LabPaq is intended for the sole use
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1) What is the purpose of ice or cold water? To cool down the gas so that it condenses and turns into a liquid. 2) when the gas collecting tube is upside down‚ give a property of this gas. It is less dense than air. 3) Why is a pencil used in drawing the origin line in chromatography? If pen was used‚ it will dissolve giving colours and so‚ the experiment won’t be accurate. Pencil doesn’t interfere with the results. 4) When using ethanol‚ give a better apparatus arrangement‚ and why? Cover
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intact while the other is broken. The different tests that are used in this experiment are : biuret test‚ Sakaguchi test‚ ninhydrin test‚ xanthoproteic test‚ and Hopkins - Cole test. Casein is one of the proteins found in milk belonging to a group called phosphoproteins. It is commonly used as a binding agent in foods. It can easily separated from milk through the introduction of acetic acid. Biuret test is a color reaction test used in determining the presence of peptide bonds in the protein
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We then went to see if the sucrose entered the bag unfortunately the benedict’s solution showed negative results. The second test ( Bag: 5% ovalbumin Beaker: 1 M sucrose) we put biuret in a test tube with the beaker solution and the indicator showed negative results. Then for the next test (Bag: 1 M Glucose Beaker: distilled H2O) they put Benedict’s solution in a test tube with the beaker liquid. The benedicts showed positive results
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I. Abstract The common organic compounds provide us with nutrients are sugars and starches‚ carbohydrates‚ proteins‚ and lipids. In this lab‚ qualitative tests are performed to identify the presence of organic compounds in food using indicators‚ chemical substances that react in a certain way when a particular substance is present. We put one indicator into the each test tube containing different solutions at a time to see what organic compound is present. The color change in the indicator tells
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is water. The remaining 10 % is made up of other substances such as reducing sugar. (a) Describe how you could safely test a sample of albumen for reducing sugar. [4] (b) A student tested some albumen for the presence of protein using Biuret reagent. The solution changed colour. It was a positive result. Describe this colour change. [1] © UCLES 2013 0610/61/M/J/13 www.maxpapers.com 3 (c) Fig. 1.2 shows an experiment to investigate the effect of acid on albumen. 3 drops
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