All-in-1 is a company that have been selling macronutrient products for the past 6 years‚ and recently they’ve been getting accused of that they have not included the important macronutrients in one of their products; the meal replacement drink. The nutra-chek company‚ takes samples of food products into their labs to test for macronutrients in the samples. The purpose of this lab is to test for four macronutrients: Monosaccharides‚ starches‚ proteins and fats. The results of this lab will determine
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Abstract The activity of invertase and the quantification of albumin and casein were performed and analyzed after extraction of the said proteins from their respective sources. Isolation of proteins was initiated by the breakage of the cell wall / membranes in three different ways. Homogenization of invertase‚ albumin and casein were achieved via grinding process‚ addition of 1M acetic acid and acidification by 0.1M hydrochloric acid correspondingly. Extraction of invertase and casein involved
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When mixed with water and the tested substance‚ a positive result will occur in it being separated from the water. The objective of the Protein Test was to test for proteins present in a substance using the Biuret Solution. The substance should produce a violet color within 10 drops of Biuret. The objectives of the Spit Lab were to test for starch‚ a reducing sugar‚ and effect of amylase on a cracker that is positive for starch and negative for a reducing sugar. Iodine would test if starch was present
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Centre Number Candidate Name Candidate Number International General Certificate of Secondary Education CAMBRIDGE INTERNATIONAL EXAMINATIONS BIOLOGY PAPER 6 Alternative to Practical 0610/6 MAY/JUNE SESSION 2002 1 hour Candidates answer on the question paper. No additional materials are required. TIME 1 hour INSTRUCTIONS TO CANDIDATES Write your name‚ Centre number and candidate number in the spaces at the top of this page. Answer all questions. Write your answers in the
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Introduction The objective of this experiment is to identify if the 12 provided solutions contain starch‚ glycogen‚ reducing sugars or proteins. The four major biological macromolecules are carbohydrates‚ lipids‚ proteins and nucleic acids. Abundant elements in the world such as carbon and nitrogen bond in different ways to form many different molecules. The functional groups are what determine their characteristics. During the experiment‚ there will be 3 test. The iodine test is completed to
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blue. (+) | 10 drops honey solution | Bright orange‚ an abundance of reducing sugars. | Yellow and clear. (-) | Unknown #4 | Reddish orange‚ an abundance of reducing sugars. | Yellow. (-) | Table 6.2 Solutions and Color Reactions for the Biuret Test for Protein Solution | Color | 2 mL egg albumen | Dark violet (+) | 2 mL honey | Yellow (-) | 2 mL amino acid solution | Pale blue (weak +) | 2 mL distilled water | Pale blue (weak +) | 2 mL protein solution | Violet (+) | Unknown
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EXTRACTION AND CHARACTERIZATION OF PROTEINS Abstract Different techniques and principles for protein extraction and characterization were demonstrated in this experiment. Various proteins were extracted from different sources: 1.67 g yeast invertase‚ 1.03 g egg white albumin‚ and 5.15 g of milk casein. Activity assay for invertase was performed using Benedict’s test and the enzymes inverting action on sucrose was confirmed. Warburg-Christian Method and Bradford Assay were also employed to determine
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RNA Analysis 1. The color result is light brown. 2. A positive Biuret test indicates the presence of proteins in the filtrate. Biuret is used to test for proteins. It is a light blue color and changes to purple in the presence of protein‚ a polymer made of amino acids. It will not change color when only amino acids are present. 3. What type of molecules present in the suspension is hydrolyzed by the mild acid treatment? 4. The products of hydrolysis are peptones‚ peptides‚ and amino acids
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Digestion Lab Abstract – The purpose of this lab was to understand how different solutions played a role in the digestion protein. By looking at different variables‚ such as temperature‚ and pH we’re capable of understanding just how certain substances functioned and when they didn’t. The data for all labs are clear and concise and give a clear understanding of what solutions work best. All three labs were placed in a warm water bath set at 37’C to stimulate the reaction as if it were taking place
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Analysis: Which sample do you suspect has diabetes mellitus? Provide your reasons. Diabetes mellitus is a condition created by the inadequate secretion of insulin from the pancreas. Insulin is a hormone that allows your body to use carbohydrates or store them for future use. Inadequate amounts of insulin causes a condition called hyperglycemia‚ which is an excessive amount of glucose in the blood stream. When the blood is filtered through the kidney‚ a large of amount of glucose is retained in the
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