unrelated proteins (Blocking phase) 3. Specific antibody (against that specific antigen) added. If quantification is needed‚ varying dilutions of the antibody are added in series. This antibody is called primary antibody. 4. Tertiary reagent added. This reagent usually consists of antibodies (labelled with enzyme) that will bind specifically to the bound antibodies in each well. This antibody is called secondary antibody. (Labelling phase) Bio-Rad Protein Assay The Bio-Rad Protein
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Macromolecules Lab Purpose: to determine a method of testing for macromolecules. Materials: Knowns: Unknown: Test Solutions: Glucose solution Unknown solution Iodine solution Gelatin solution Benedict’s solution Starch solution Biuret solution Oil Brown paper Water Procedure: 1. Create a data table. 2. Label 5 test tubes with known solutions. 3. Add 10-20 drops of each known solution to respective test tubes‚ do not mix pipets! 4. Add 3-5 drops of a test solution
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|Molisch reagent | |agar-agar‚ gum arabic‚glycogen‚ cotton‚ |I2 in KI solution (Lugol’s iodine reagent) | |starch |Benedict’s reagent | |10% HOAc solution‚ |Barfoed’s reagent
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Antony R 9:00Am-11Am Unknown Number 94 Unknown Results Streptococcus salivarius The first step in my unknown identification was to carry out a gram stain to observe the cell shape and arrangement. My unknown turned out to be gram positive bacteria cocci shaped with long chain cell arrangement. The unknown was gram positive because the cells stained blue-violet ‚this indicate that my unknown has ability to retain crystal violet-iodine after alcohol application on the cells which is a gram
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5) Provide the structure of the major organic product in the reaction below. (CH3)2CHCH2CH2CHO → 6) Provide the structure of the major organic product in the reaction below. 7) Provide the reagents necessary to carry out the transformation shown below. 8) Provide the reagents necessary to carry out the transformation shown below. 9) 2-Methylpentan-3-ol is classified as __________. A) a primary alcohol B) a secondary alcohol C) a tertiary alcohol D) a phenol
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FOUNDATION OF MEDICAL SCIENCE BIOLOGY-FGS0044 LAB REPORT 3.1 : CARBOHYDRATE DETECTION TEST LAB REPORT 3.2 : PROTEIN DETECTION TEST LAB REPORT 3.3 : LIPID DETECTION TEST GROUP MEMBERS : CONTENTS NO. | TITLE | PAGE | 1 | INTRODUCTION | 3 | 2 | LAB 3.1 : CARBOHYDRATES DETECTION TEST | 5 | 3 | LAB 3.2 : PROTEINS DETECTION TEST | 8 | 4 | LAB 3.3 : LIPIDS DETECTION TEST | 12 | 5 | REFERENCES | 14 | INTRODUCTION Carbohydrates (saccharides) are molecules consist of carbon
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the reagent used to prepare the standard. Reagents used as standards are divided into primary reagent and secondary reagent. A primary reagent can be used to prepare a standard containing an accurately known amount of analyte. A primary reagent must have a known stoichiometry‚ a known purity (or assay) and be stable during a long term storage both in solid and solution form. The purity of a secondary reagent in a solid form or the concentration of a standard prepared from a secondary reagent must
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minutes. Cool and filter the mixture. Add enough fresh 2M HCl to wash the residue and then bring the volume of the filtrate to 5ml. Place 1ml each of the filtrate to 2 test tubes as follows: 1. To 1ml of the filtrate‚ add a few drops of Mayer’s Reagent. Result: Light brown solution with
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two new σ bonds are formed. • Alkenes are electron rich‚ with the electron density of the π bond concentrated above and below the plane of the molecule. • Because alkenes are electron rich‚ simple alkenes do not react with nucleophiles or bases‚ reagents that are themselves electron rich. Alkenes react with electrophiles. Electrophilic Addition Energy Diagram: • The mechanism of electrophilic addition consists of two successive Lewis acid-base reactions. In step 1‚ the alkene is the Lewis
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–NH2 to give a purple colour. There are three samples in the experiment: sample A‚ Sample B and Sample C. The sample A contains 2 distilled water and ninhydrin reagent. There are egg white and ninhydrin reagent in sample B. Succinylated egg white and ninhydrin reagent were in sample C. Because distilled water cannot react with ninhydrin reagent‚ the sample A was as a blank in the experiment. We measured the absorbance of the egg solution at 570nm. In sample B‚ the native egg white was added. The data
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