To determine protein concentration in apple juice. Procedure Part1 7 test tubes were prepared and labeled. The appropriate amount of BSA and distilled water were pipetted into the respective test tubes according Table 3.1. 500µl of Branford Reagent were added into all the test tubes. The test tubes were all vortexed and mixed.
Premium
Synthesis and Structure of Alcohols Alcohols can be considered organic analogues of water. H O H R O H Alcohols are usually classified as primary‚ secondary and tertiary. H R H OH H primary R R OH R R OH OH R secondary tertiary phenol Alcohols with the hydroxyl bound directly to an aromatic (benzene) ring are called phenols. Nomenclature of Alcohols (Normally any compound’s name which ends in –ol is an alcohol of some sort) IUPAC rules that:
Premium Alcohol
Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state. When protein is bound to the dye the color turns blue. In this experiment a standard was used to start the experiment‚ specifically the BSA stock solution. This standard
Premium Amino acid Coomassie
of one of the ions or anions combined with one of the reagents such as NaCl. It was found that it could be separated out after centrifuging and would result in a sample without the ions or anions if it precipitated. With the data from week one a method of adding the reagents in a certain order would result in the ability to separate all ions or anions out of a solution. Overall‚ knowing how the cations or anions reacted with certain reagents benefited greatly to determine a way to create a separation
Premium Chemistry Solubility Separation process
addition of a Grignard reagent to an aldehyde/ketone = stable tetrahedral intermediate (alkoxide) - addition of an alcohol to a carbonyl group in the presence of a base = unstable intermediate (hemiacetal/hydrate) - nucleophiles with good leaving groups (anions i.e. Cl-‚ RO-‚ RCO2-) = unstable - starting carbonyl compound with good leaving group = unstable (makes a Tetrahedral intermediate then collapses to form the starting carbonyl group) i.e. Grignard reagent added to an ester
Premium Alcohol Hydrogen Oxygen
ferric chloride‚ Bariit’s A and B solutions‚ Kovac’s reagent‚ zinc dust‚ hydrogen peroxide‚ API 20E incubation tray‚ Bunsen burner‚ oxidase reagent and bacteria Methods: The method used for the API (analytical profile index) 20E System was the aseptic technique was used for the inoculating loop to get a unknown colony from the streak plate or pure culture slant. A small amount was smeared over the filter paper and several drops of oxidase reagent were added and the color change was noted. Another
Premium Hydrogen peroxide Bacteria Amino acid
Lesson 1 Key Questions 1. Mg2+ has 10 electrons 2. Given that the half life of the radioisotope carbon -14 is 5730 years‚ it would not be useful in dating bones that are over a million years old. After 40 000 years of age less than 1% of the 14C is left in the bone and thus it is not useful for determining the exact age beyond that. 3. Hydrolysis - A chemical reaction where a chemical is broken down by a reaction with water. Hydrolysis is important for the digestion of food‚ making
Premium Carbon Isotope Proton
| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing
Premium Staining Gram staining Enzyme
ammonia and vortex. - Complexing reagent is added (0.8ml) and vortex. Cathecol: - 0.5ml is taken from sample‚ standard and control. - 50µl internal standard is added‚ then vortex. -13µl ammonia is added‚ then vortex. -Complexing reagent is added (0.8ml)‚ then vortex. Solid phase extraction (SPE) MET
Premium Chemistry Oxygen Water
positive. Lieberman-Burchard is a reagent used in a colorimetric test to detect cholesterol‚ which gives a deep green color. This color begins as a purplish‚ pink color and progresses through to a light green then very dark green color. The color is due to the hydroxyl group (-OH) of cholesterol reacting with the reagents and increasing the conjugation of the unsaturation in the adjacent fused ring. Because this test uses acetic anhydride and sulfuric acid as reagents caution must be exercised so as
Premium Cholesterol Green