we routinely use for the transfection of mammalian cells [4]‚ but it was possible to quantitate other PEIs (diVerent molecular weights; branched) with this method (data not shown). The mixing of a PEI solution with the Advanced Protein Assay (APA) reagent (Cytoskeleton‚ Denver‚ CO‚ USA) resulted in the formation of a colored
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Experiment Four: Organic Synthesis of Aspirin Abstract The purpose of this experiment is to synthesize a common organic product called acetylsalicylic acid (aspirin)‚ and to become familiar with the optimum conditions needed for successful yields. Aspirin is produced from an acid catalyzed reaction between salicylic acid with acetic anhydride. The crystalline aspirin is synthesized and purified by recrystallization‚ although there is not a hundred percent yield due to sources of error. Introduction
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Microbiology-2460 Lab-003 March 31‚2008 Lab Report-Escherichia coli Abstract The purpose for this lab report was to identify and inform of an unknown bacteria that has been causing a patient to have lower abdominal and pelvic pain. To obtain the identification of this unknown bacterium‚ several biochemical tests needed to be performed in order to prescribe the correct medication to treat and cure the symptoms. Introduction In a lab today‚ I am to identify an unknown bacterium that is
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LMounika Alluri Chemistry HL Block D 19/9/12 LAB REPORT #1 LIMITING REAGENTS INVESTIGATION Aim: To determine the limiting reagent and percent yield of the reaction between potassium iodide with lead (II) nitrate solution. Apparatus required: Safety glasses‚ funnel stands‚ watch glass‚ oven‚ electronic balance‚ wash bottle with distilled water‚ test tubes‚ 10.0mL 0.50M lead (II) nitrate‚ 10.0mL 0.30M of potassium iodide solution‚ two 100.0 mL beakers‚ funnel‚ filter paper. Reaction
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Main aim of experiment 5 is to use the biuret test to quantify the concentration of the Bovine Serum Albumin (BSA) then constructing a standard curve graph and to use the spectrophotometer to perform an enzyme assay using different concentration of the BSA. Experiment 5 also verifies the Beer-Lambert Law‚ which is the linear relationship between absorbance and concentration of an absorbing species. Absorbance formula is shown in fig. 1.1. However‚ the Beer-Lambert Law is not obeyed at high concentration
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Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple) or LPS and PG (- Pink) Primary Stain- Crystal Violet- Stains Cells Purple Binding Agent- Mordant (Iodine)- Fixes C.V. into Cell Wall of G+ cells Decolorizing Agent-
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a framework for evaluating different chemistries‚ and an ideal to strive for in new reaction chemistry (1‚2‚3). Green Chemistry Principle: Atom Economy Atom economy means maximizing the incorporation of material from the starting materials or reagents into the final product. It is essentially pollution prevention at the molecular level. For example‚ a chemist practicing atom economy would choose to synthesize a needed product by putting together basic building blocks‚ rather than by breaking down
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endpoint than phenolphthalein. Te equivalent weight can be calculated from the following equation: B. ALCOHOLS 1. Ceric Nitrate – A qualitative test for alcohols and phenols. a. For Water-Soluble Compounds Add 0.5 mL of the ceric nitrate reagent to 2-3 mL of distilled water in a test tube‚ and mix thoroughly. Add 1 to 2 drops of the compound to be tested‚ shake‚ and note the color. If the
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Transfection for HMECs using Lipofectamine LTX + Plus reagent NB: • Using PLUS™ Reagent (Cat. No. 11514-015) enhances transfection performance in HUVEC cells. • The addition of antibiotics to media during transfection may result in cell death Transfection of HMECs Use this procedure to transfect plasmid DNA into HMECs cells in a 12-well format All amounts and volumes are given on a per well basis. 1. Cell density should be 50~80% confluent on the day of transfection (use the normal growth
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perform approximately 60 prostate biopsies a year. Free PSA (cost activity / object) is available on equipment that we currently use under a reagent rental agreement (we pay only for reagents‚ as the cost of the equipment is rolled into the cost of the reagents). The vendor has quoted a price of $8.00 per test (not $8.00 per billable test) for free PSA reagents. Issues to consider in evaluating the profitability of bringing the test ( Free PSA ) in house:1.Effect on direct cost. 2.Possible increase
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