Nucleophillic Substitution Reaction Introduction: This lab experimented on the reactions of the chemicals that undergoes SN1 or SN2 reaction. Nine different compounds were given to be examined with two reagents - NaI in acetone and AgNO3 in ethanol. The SN1 reaction happens in AgNO3 in ethanol reagents‚ and SN2 reaction is in NaI in acetone. Procedure: Following the lab manual. Results: compound NaI /acetone (SN2) AgNO3/ethanol (SN1) Bromobenzene NR NR Bromocyclopentane X 2 sec
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Objectives 1. To prepare a standard curve of absorbance versus protein concentration by using Bovine Serum Albumin (BSA) 2. To determine BSA concentration in two sample solutions 3. To determine protein concentration in apple juice. Materials Bradford reagent‚
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similarities between this group of elements. Hypothesis: Those elements in the same row will exhibit different properties while elements in the same columns will exhibit the same properties. Materials: 24-well chem plate or plastic sheet reagents 0.1 M K4Fe(CN)6 0.1 M KSCN 0.1 M NaCl 0.1 M Na2SO4 0.2 M (NH4)2C2O4 1.0 M HNO3 thin-stemmed pipets or dropper bottles for solutions‚ 10 transition elements 0.1 M Cu(NO3)2 0.1 M Fe(NO3)3
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| | H R R Primary Alcohol(1 ̊) Secondary Alcohol(2 ̊) Tertiary Alcohol(3 ̊) Lucas reagent‚ a mixture of concentrated hydrochloric acid and anhydrous zinc chloride‚ can be used to differentiate the three classes of alcohols. Tertiary alcohol turns cloudy or appears in two layers almost immediately‚ secondary alcohol reacts within 5 to 10
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Pre-lab 1: Identifying Carbohydrates Abstract The point of this lab is to determine if a substance contains carbohydrates such as a reducing sugar and/or polysaccharides. This will be done by using Benedict’s reagent and Iodine stain tests. Benedict’s reagent will react to reducing substances in the solution by oxidizing it and changing the structure of the reducing sugar to form a colored precipitate. The color of this precipitate can be used to determine the concentration of reducing sugars in
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of the protein wherein the secondary‚ tertiary and quaternary structures are all affected. Denaturation is brought about by various kinds of physical and chemical means; this includes the addition of strong acids‚ heavy metal cations‚ alkaloidal reagents‚ salting out and addition of organic solvents. Precipitation is commonly observed when a protein is denatured. The experiment was done to explain the principles behind each method of denaturing proteins. In addition of strong acids to protein solution
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In Vitro Diagnostic (IVD) Market [Instruments‚ Reagents & Data Management Systems] [Technique (Immunoassay‚ Clinical Chemistry‚ Molecular Diagnostics‚ Hematology) & Applications (Diabetes‚ Infectious Diseases‚ Cancer & Cardiology)] Systems‚ End Users] Forecast To 2017 On 13rd June 2014 The report focuses on "In Vitro Diagnostic (IVD) Market [Instruments‚ Reagents & Data Management Systems] [Technique (Immunoassay‚ Clinical Chemistry‚ Molecular Diagnostics‚ Hematology) & Applications (Diabetes
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the carbohydrate by concentrated sulfuric acid to produce an aldehyde which condenses with two molecules of phenol‚ resulting in a coloured compound. Molisch reagent is a solution of α-napthol in 95% ethanol. The aldehydes produced are pentoses and hexoses which can derive to furfural and hydroxymethyl furfural respectively. The Molisch reagent further reacts with furfural to yield a coloured product which is a deep violet ring at the interface of two layers. The reaction occur at the junction where
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2012 Aim The aim of this lab is to determine the order of the reaction between Propanone and aqueous Iodine with respect to Iodine. Apparatus and Reagents Apparatus I. Eye Protection (1) II. Spectrophotometer III. Cuvettes for spectrophotometer IV. Burette (4) V. Burette Stand (4) VI. Stopwatch VII. Conical flask (2) Reagents I. 0.020M I2 (aq.) II. 2.0M CH3COCH3 (aq.) III. 2.0M HCl (aq.) Raw Data Absorbance of I2 Solution Volume of I2 Solution/ cm3
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amylase is working. If the amylase is inactivated‚ it can no longer hydrolyze starch‚ so the blue color of the starch-iodine complex will persist. You will also test for the presence of glucose in the samples using Benedict’s reagent. When a blue solution of Benedict’s reagent is added to a glucose solution‚ the color will change to green (at low glucose concentrations) or reddish-orange (at higher glucose concentrations). Starch will not react with
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