OSMOSIS LAB BACKGROUND INFORMATION. Dialysis tubing is being used to model a plasma membrane. Isotonic solutions are solutions that have the same concentration of solutes on both sides of the membrane. Hypertonic solutions are solutions that have more solute to the one with less solute. Hypotonic solution are solutions that have less solute to the one with more solute. RESEARCH QUESTION:which dialysis tubing will gain the distilled water and which one will loss its content
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of Vanillin. Stock solution of vanillin in water with concentration of 50 ppm was prepared. Four solutions with concentration of 5 ppm‚ 1 ppm‚ 0.5 ppm and 0.1 ppm were made from stock solution. UV-Vis spectra of standard solution was recorded at 229 nm and equal to 0.544 AU. Vanillin was separated on SPE cartridges using a 50% methanol and 50% water as eluting solvent. UV-Vis of blank‚ 1 ppm and 0.1 ppm vanillin sample was obtained at 0.078 AU and 0.014 AU. 5 ppm solution of amlodipine besylate
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Research Question: In this lab‚ our goal was to look at the effects of different sucrose concentrations on osmosis in potatoes. Our research question was “How do the sugar levels affect the rate of osmosis in potatoes?”. Background Information: Solutions consist of solutes dissolved in a solvent. In all living organisms there are many different types of solutes including salts and sugars. The major solvent is water. There are different concentrations of solutes in various regions of an organism
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Results The Malpighian tubules were incubated in the solutions for 15 minutes before being observed. The transport of CPR into the lumen was highest when there was no addition of oligomycin‚ ouabain and p-aminohippurate (PAH). The least transport occurred from the addition of ouabain and PAH (table 1). There was a strong positive correlation (R2=0.9881) between increased potassium concentration in the CPR solution and the transport of the solution into the tubule (figure 1). The least tubule transport
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Objective: The objective of this lab was to find and examine the viscosities of ideal and non-ideal solutions. The ideal being the toluene/p-xylene and the non-ideal being the methanol/water. The second objective of this lab was to investigate the temperature dependence of viscosity (Halpern‚ 17-1). Introduction: Viscosity is the resistance to flow of a certain fluid. In this experiment two solutions are used. According to the definition of viscosity mobile liquids have a relatively low viscosity
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solvent. This experiment is specifically designed to measure the solubility of sodium hydroxide in waters of different temperatures.! ! Hypothesis! Higher temperature of water allow the particles of sodium hydroxide(s) more mobility between the solution and their solid state because the particles are absorbing energy from their surrounding. Therefore‚ higher the temperature of water‚ higher the solubility of sodium hydroxide. ! ! Table of variables! Variable measured Dependent Variable
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Lab Report #1 Introduction A cell’s plasma membrane is known to be selectively permeable. This implies that the membrane is selective on what substances can pass in and out of the cell. There are two methods of transport that occur through the plasma membrane. One method of transport is called active process which uses ATP energy to transport substances through the membrane. The other method is called passive process which does not require the use of ATP energy. During passive processes
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examine the effect different concentrations of sucrose solution has on the movement of water across the cell membrane. Hypothesis: That the potatoes will either increase or decrease in mass‚ due to the effect of the different concentrations. Osmosis will occur. Apparatus: * Fresh potatoes * Apple corer * Scalpel * Petri dishes X 10 * 50ml measuring cylinder * Distilled water * Paper towelling * Sucrose solutions: * 1.0M * 0.8M * 0.6M * 0.4M *
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across the membrane in one direction than the other (Hayden and McNeil 2012). During osmosis the net movement of water molecules will be from a solution that has a lower osmotic concentration to a solution that has a higher osmotic concentration. When a solution has a higher concentration of solute within the cell than out‚ it is called hypertonic. When a solution has a lower concentration of solute within the cell than out‚ it is called hypotonic. And when there are equal concentrations inside and out
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of a solution of a given compound and its concentration. This is normally expressed as . A = Absorbance measured = Absorptivity C = Molar concentration L = Length of cuvette Equipment: • Gilson pipette • Eppendorf tubes • Copper sulphate • Spectrophotometer • Cuvettes • Distilled water Method: 1. Use a Gilson pipette to measure out five different solutions of copper sulphate and distilled water. 2. Pour into a 1.5ml test tube‚ label each tube 1 to 5 to indicate each solution and place
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