The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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and Absolute Zero Determination Lab Report Experiment 4 Introduction In this experiment we measured the relationship between temperature‚ volume‚ and pressure. Objectives: Conduct a set of experiments‚ each of which illustrates a gas law. Gather data to identify the gas law described by each activity. Complete the calculations necessary to evaluate the gas law in each activity. From your results‚ derive a single mathematical relationship that relates pressure‚ volume‚ and temperature. Hypothesis:
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Introduction This report will investigate the six essential nutrients required in our diet. The report will provide the function of each nutrient and explore why they are vital for the body. Carbohydrates Carbohydrates are the most important nutrient for the body as it is the largest possible source of energy. Carbohydrate supplies the body with energy‚ which allows the brain to function more effectively and allows organs to operate sufficiently. When the body has energy this allows each
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Heart rate in black worms with and without added glucose Introduction: The circulatory system is important in circulating blood‚ nutrients‚ oxygen and chemicals to the body. A circulatory system commonly consists of the fluid it contains‚ such as blood‚ a pumping vessel such as a heart‚ and the vessels‚ which carry the fluid through the body. In a closed circulatory system‚ the fluid‚ blood‚ is contained within the vessels as it circulates through the body. The Lumbriculus variegates‚ or
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A. Goal and Purpose: Session 1: In this lab‚ we will achieve a simple Friedel-Crafts alkylation of anthracene. The choice of anthracene as an aromatic substrate stems from two considerations. First‚ there is a question of regioselectivity. Second‚ anthracene and its derivatives are highly visible under UV light. Session 2: In this lab‚ we will complete a partial conversion of 9-acetylanthracene using m-chloroperoxybenzoic acid (mCPBA). We will also determine by NMR‚ the regiochemistry of the
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helps to regulate blood glucose levels within the body‚ releasing insulin when levels are high and glucagon when levels are low. In this experiment‚ we were interested to see how exercise impacted the body’s
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Lab Report: Understanding Photosynthesis Gen Biology Lab Abstract: This lab was called photosynthesis: understanding photosynthesis. It is a highly complex process that needs to be broken down in many steps to understand how it works. This lab covers the big components in photosynthesis including carbon dioxide intake‚ light consumption‚ and varying pigmentation. Introduction: Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special
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Labs and Diagnostics Colonoscopy This test allows direct visualization of the small bowel‚ rectum‚ and colon. It is recommended for patients who have experienced fecal occult blood or obvious blood in the stool. It is also recommended for patients who have experienced a change in bowel habits or are experiencing abdominal pain. In patients who have had colorectal cancer‚ polyposis‚ or inflammatory bowel disease it is used to observe for continued progression of their condition. During the procedure
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IntroductionThe purpose of this experiment is to determine whether or not caffeine has an affect on the heart rate of DaphniaHypothesisThe Daphnia heart rate will increase with the concentration of caffeine. I based my hypothesis on the fact that humans heart rates increase with caffeine as it acts as a stimulant drug. In the experiment there will be several variables that I need to try and control‚ the temperature of the water is one of these because if the temperature is higher or lower on the
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Problem: Does prior exercise affect the ability to squeeze a clothespin? Hypothesis: After exercise‚ people will be able to squeeze the clothespin faster than those without prior exercise. Materials: For this experiment a timer and a clothespin were used. Methods: To begin this experiment an individual was asked to squeeze a clothespin as many times as possible within one minute and the results were recorded. Then this same individual was asked to exercise (run in place‚ preferably)
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