Blue white selection is a widely used method in screening recombinants in cloning. This is based on the gene product of lac z gene. The plasmid vectors contain this gene which produces β galactosidase enzyme. When a gene is inserted close to lac z gene‚ the reading frame will be distorted and the gene is inactivated. So the transformed cells will not produce this enzyme and are called competent cells. After the recombination‚ the bacterial cells are grown in a medium containing X gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside)
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“red” oceans. Blue Oceans in contrast‚ denote all the industries not in existence today-the unknown market space untainted by competition. In blue oceans‚ demand is created rather than fought over. There is ample opportunity for growth that is both profitable and rapid. In blue oceans‚ competition is irrelevant because the rules of the game are waiting to be set. Blue Ocean is an analogy to describe the wider‚ deeper potential of market space that is not yet explored. Like the “blue” ocean‚ it is
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child‚ to the death of a parent; things in life that impact us with such unrelenting force‚ that we are unable to even fathom their depths. It is the job of a writer or poet to make a reader feel the emotions of others‚ describe the indescribable‚ and tell a story. W. H. Auden’s “Funeral Blues” does all three‚ and more for readers‚ due to its beautiful descriptive language‚ blinding imagery‚ and theme. The poem demonstrates everything that is needed in such a fine piece of literature. Have you ever
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Conflict and Characterization in “Sonny’s Blues” “Sonny’s Blues” is a short story by the author James Baldwin. It is a story about two brothers and `their severe struggles in Harlem and the different ways in which they handled them. The author shows through the narrator and his brother Sonny how two individuals can follow two totally different life paths‚ be distant for most of their lives‚ but in the end find common ground through a shared understanding of the pain of human struggle‚ which this
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Exploring the Dark Side: Contrasting Themes in "Blue Velvet" The subconscious psyche is one of the most fascinating and almost completely inexplicable aspects of human behavior. Even more intriguing than merely the subconscious is the notion of a darker‚ more repressed side that many individuals refuse to acknowledge exists within them. In David Lynch’s film "Blue Velvet‚" the director attempts to explore the psyche of a young man named Jeffrey Beaumont‚ most notably the clash between his darker
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Analysis of Operation Blue Star and its effects on the Gandhi dynasty The period of time including and following Operation Blue star is considered a dark time in India and black spot in Indian history. It is a time Indian would rather forget‚ yet still to this day debate about. Was Indihar Gandhi correct in instating operation Blue Star‚ inflicting damage to the Golden temple‚ and being responsible for the killing of anywhere from 492 (official reports) to 1500 (estimates run as high as) civilians
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Blues of Career Building Actually‚ we are surrounded with polluted milieu that compiles of a lot of abortive feelings such as financial scarcity‚ impractical depression and blues of career building. Though financial constraints and depression are the vicissitudes of life‚ but blues of career building matters very much. In fact‚ blues of career affect our entire life. Analytically‚ if we see a person’s career building has been very tough now-a-days. Building a career is difficult
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Communication Break Down V. Budget Control VI. Risk Management VII. Measurement 4. CONCLUSION ……………………………………….................20 5. BIBLIOGRAPHY …………………………………….............….21 1. EXECUTIVE SUMMARY The Blue Spider Project is an example of how a project manager incorrectly balanced the staff and work‚ resulting in a poor performance from a project. We see that Parks Corporation bidding to be part of a government contract and it shows problems with communication
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Blue ocean strategy 1. Brief introduction 2. Quotes and examples 3. Personal opinion and critics 1. Introduction This book is an introduction to blue ocean strategy. Strategy management is here divided in the two; the red and the blue strategy. First let’s talk about the red ocean‚ red for blood‚ where companies can be compared to sharks eating each other by competing on existing markets with unoriginal strategies especially by competing on price and try to get a bigger market share
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through a country‚ my backpack would contain an American flag‚ a toy Maryland blue crab‚ and my diploma. The American flag represents not only my love for America but also my unique identity as an American. The blue crab symbolizes the unique culture of Maryland and my connection to the state of Maryland. The diploma is a symbol for not only the senior class but also my second family. The American flag‚ the Maryland blue crab‚ and the high school diploma are symbols that represent who I am. I selected
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