µL of the freshly prepared HRP substrate solution was added to each well. The HRP substrate solution25 was 21 ml citrate buffer (220 mM sodium citrate monobasic‚ pH 4.0) + 8.1µL H2O2 (30%) + 525 µLTMB solution (40 mM TMB‚ 8 mMtetrabutylammonium borohydride‚ in N‚N-Dimethylacetamide). The microtiter plate was incubated for 30 minutes. Eventually‚ the color development was stopped by adding 100µL of 1 M H2SO4. And therefore‚ the absorbance was measured using plate spectrophotometer SpectraMax Plus384
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Designation: D2972 – 08 Standard Test Methods for Arsenic in Water1 This standard is issued under the fixed designation D2972; the number immediately following the designation indicates the year of original adoption or‚ in the case of revision‚ the year of last revision. A number in parentheses indicates the year of last reapproval. A superscript epsilon (´) indicates an editorial change since the last revision or reapproval. 1. Scope 1.1 These test methods2 cover the photometric and atomic
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Preparation In a dry round bottom flask‚ 1.5mL of cyclohexanone was added with 5mL of methanol. The solution was cooled in an ice bath for 3 minutes after which 0.2g of sodium borohydride was measured and added to the solution. Upon mixing‚ a gas was formed (bubbles). The round bottom flask was removed from the ice bath and placed at room temperature. After 10 minutes at room temperature‚ 5.0mL of 3M NaOH and 2.0mL of distilled water was added to the solution. Isolation and Characterization The
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Using Selective Media to Monitor the Fermentation Process of Cabbage and Cucumber‚ for Fourteen Days Abstract: Introduction: The goal of the ecological succession lab is to demonstrate succession with the fermentation of cabbage and cucumbers. The cabbage will ferment into sauerkraut and the cucumber will ferment to pickles during fermentation process that will changes the species structure and the community of time. During the fermentation process we watched the pH become more acidic‚ since
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Chapter 17: Alcohols and Phenols C O O H H sp3 alcohol pKa~ 16-18 phenol (aromatic alcohol) pKa~ 10 Alcohols contain an OH group connected to a saturated carbon (sp3) Phenols contain an OH group connected to a carbon of a benzene ring O H C C H C C O chemistry dominated by the keto form 76 enol keto H O H R O H R O R R O O R’ water alcohol R S H ether R S R peroxide R S S R’ thiols thioether disulfides
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Panther ID: NAME: FLORIDA INTERNATIONAL UNIVERSITY Department of Chemistry CHM 2211-U02– ORGANIC CHEMISTRY II PRACTICE EXAM I CHM 2211-U01 SAMPLE EXAM I‚ Page 2 Type of Vibration C-H Frequency (cm-1) 3000-2850 1450 and 1375 1465 3100-3000 1700-1000 3150-3050 1000-700 ca. 3300 2900-2800 2800-2700 1680-1600 1600-1400 2250-2100 1740-1720 1725-1705 1725-1700 1750-1730 1700-1640 1810 and 1760 1300-1000 Intensity s m m m s s s m w w m-w m-w m-w s s s s s s s Alkanes -CH3 -CH2Alkenes
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important in a variety of different industries. This post will look at some of the common uses of aluminum in the world today. Uses of Aluminum * Aluminum metal is very useful for packaging. It is used in cans and aluminum foil. * Aluminum borohydride is added to jet fuel. * Electrical wiring is sometimes made from aluminum or a combination of aluminum and copper. * Hydrogen gas‚ an important fuel in rockets‚ can be obtained by reacting aluminum with hydrochloric acid. * Super purity
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Introduction to Alkane Nomenclature A. Determining the Priority of Functional Groups. What’s in a name? 3-ethyl-5-(1-methylpropyl)-4‚4-dimethylnonane Too big a subject to cover on one sheet! This paper will focus on alkanes. Determining functional group priority will be the subject of a subsequent sheet. suffix http://masterorganicchemistry.com D. Applying the Lowest Locator Rule F. Dealing With Branched Substituents (the IUPAC Way) Number the chain from one end so as
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CHM 138H INTRODUCTORY ORGANIC CHEMISTRY I TERM TEST II March 18th 2010 TOTAL MARKS: 70 NAME (PRINT): ___________________________ Student #: ______________________ TUTORIAL GROUP #: ___________ Answer all questions in the spaces provided on the accompanying answer sheet. It is attached to the back of the test paper. You may detach the answer sheet. Nothing on this question sheet will be marked. PRINT YOUR NAME‚ STUDENT NUMBER‚ and TUTORIAL GROUP NUMBER on this test
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Chemistry 3373F Lab Manual 2008 Modified 11/07 Table of Contents Chem 3373 Laboratory Schedule for Fall 2008.............................................................................2 The Benzoin Condensation of Benzaldehyde ..............................................................................3 Synthesis of Dilantin and Related Compounds (two weeks).........................................................6 Synthesis of an Alkaloid: Pseudopelletierine (two weeks) .............
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