Standard Curve Raw Data (Figure 1) Standard Curve-Corrected Data (Figure 2) The figures‚ 1 and 2‚ show the relationship of Bovine Serum Albumin concentration and Absorption of light at 595 nm. Figure 1 shows the standard curve of Raw Data and figure 2 shows the standard curve- Corrected Data. Bovine Serum Albumin or BSA was weighed and dissolved in water to achieve a certain stock concentration. Water was used as a diluent. Quantitative dilutions of this stock
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gouvernement Interveners: British Government and European Commission FACTS: In 1996‚ the bovine spongiform encephalopathy (BSE) is a progressive neurological disease‚ widespread in the United Kingdom. After that a link between this and a variant of a particular disease affecting human beings ( Creutzfeldt-Jacob) has been discovered‚ the European Commission adopts Decision 96/239/EC prohibiting British exports of bovine meat to other Member States and third countries. This Decision is based on Directive
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WHAT IS MAD COW DISEASE? -Mad cow disease‚ formally known as Bovine Spongiform Encephalopathy (BSE). -Is a chronic degenerative disease that attacks the central nervous system of cattle. -Destroying brain tissue and eventually causing dementia and death. There is no known cure. IN HUMANS -In human beings it is known as the Creutzfeldt-Jakob syndrome -A rare neuro-degenerative disease that is a contagious form of spongiform encephalitis‚ -It is thought to be caused by a slow virus which
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References: CDC - Bovine Spongiform Encephalopathy (BSE). (n.d.). Centers for Disease Control and Prevention. Retrieved May 9‚ 2013‚ from http://www.cdc.gov/ncidod/dvrd/bse/ CDC - Prion Diseases. (2010‚ January 1). Centers for Disease Control and Prevention. Retrieved May
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charged R-groups. The strongest of the four bonds is the disulfide bridge that literally holds the structure together. This bond is a covalent bond between two sulfur atoms that occurs only between two cysteine amino acids (Borges‚ 2014). F. Bovine Spongiform Encephalopathy Prions are types of proteins that do not require nucleotides to reproduces. In 1997‚ Stanley Prusiner discovered prions and theorized that they existed in two forms: cellular (PrPc); and infectious (PrPSc)‚ and that the
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is‚ again‚ to develop a standard curve for Bovine Serum Albumin‚ and this is done by preparing at least five dilutions. According to most research findings‚ scientists suggest that to determine the standard curve or concentration of an unknown sample of BSA using a quantitative spectrophotometer‚ the standard is diluted to a range from 0.01µg/ml to 10µg/ml. Material and method This particular experiment includes; spectrophotometer‚ 240µg/ml of Bovine Serum Albumin (BSA)‚ 5 test tubes‚ Coomassie
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disease lasts for 14+ months. The patient eventually falls into a coma and tragically dies. Luckily‚ the ultimate chances for humans to acquire Mad Cow Disease are very‚ very low. In fact‚ there have only been 3 reported cases in the U.S.! “BSE (Bovine Spongiform Encephalopathy).” www.cdc.gov. CDC‚ 17/3/11. Web. 6/3/12 <www.cdc.gov/ncidod/dwrd/bse> “BSE and vCJD.” www.google.com. Google‚ 1/1/97-12. Web. 11/3/12 <www.google.com/search?tbm=isch&hl=en&source=hp&biw
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Week 2 – Discussion Board Holy Cow – Blessed Bovine-For the Love of the Cow After reading “India’s Sacred Cow” by Marvin Harris (Henslin‚ James M. (2010). Life In Society: Readings to Accompany A-Down-To Earth-Approach‚ 4th Ed.)‚ I was quite intrigued to learn more about the beliefs of the Hindu religious practices in India and the sacredness of the cow. As the article states‚ “The sacredness of the cow is not just an ignorant belief that stands in the way of progress. Like all concepts of the
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The total protein content was estimated as proposed by Lowry et al. A bovine serum albumin stock solution (1mg/ml) was prepared in sodium hydroxide (1N). Five different concentrations (0.2‚ 0.4‚ 0.6‚ 0.8‚ 1 ml) of the prepared solution were taken in different test tubes. In another set of test tubes‚ 0.1 and 0.2 ml of the extract were taken. In each test tube‚ the volume was made up to 1 ml‚ followed by addition of the prepared alkaline solution (5 ml) at room temperature. The solutions were left
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extract collagen from mammals. But due to negative effects of collagen from mammals‚ fish skin serves as another option because it also contains collagen. It is much safer for it’s being said that marine collagen are not associated with the risk of bovine spongiform encephalopathy (BSE) outbreaks which is common in pig‚ cow or any mammals’ skins and bones. In extraction of collagen we can be able to produce two kinds of collagen where they only differ in solvent used in the process. Acid soluble
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