Determining the Concentration of an Unknown Protein Solution with the use of the Bradford Method Paul Alcala‚ Andrea Basa‚ Melissa Caagbay‚ Frances Cayomba Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state
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Problems/issues: • Mei Ling may not have enough money to cover her later years expenses; • She had been a fairly aggressive investor‚ but she may not want to take very risky investment since she cannot afford to lose at her age. Goals and objectives: • Cash-flow management; • Pay off the property investment loan; • Superannuation adequacy and savings programs; • Personal investing. Assumptions: • Taxation and other legislative conditions will remian stable; • Income will incerease
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Bradford Protein Assay Practical Report 1. Present your data (including raw data and calculated concentrations) for the protein standards in the form of a clear table. Give one example of how you calculated protein concentration. Do not forget a descriptive title and units (4marks) Title either too long or not descriptive or absent Your results are in duplicate & shouldn’t be referred to as ‘set1 & set2’ or ‘original’ and ‘duplicate’ Many of you think units of absorbance are nm but A has arbitrary
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CYMH 5331 Treatment Plan: Diagnosis of Client by Physician/Psychiatrist. Clinical impression by therapist Client Diagnosis (Gary): Gary’s diagnosis is insufficient sleep resulting in a circadian rhythm sleep-wake disorder. In addition‚ Gary’s situation means that he is suffering from other factors that may need clinical attention. Certain environment and psychology events because he living in a single-parent family where the sole parent works night shift and is absent for the majority of Gary’s
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Title: Bradford Assay: Creating a Standard Curve and Determining Unknown Protein Concentrations Introduction: In biochemistry the ability to determine the quantity of a certain substance in a solution is extremely important. Often protein concentration within a biological organism is essential in determining the function or condition of that organism. If the protein concentration in an environment is increased or decreased it can have detrimental side effects. If there is a decrease in hemoglobin
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The Case of Littleton Manufacturing Executive Summary Littleton Manufacturing is …. For years‚ they have been able to capitalize on synergy between the Information Technology group and other departments and look to continue that trend with the deployment of a new paperless job posting system. A similar transition to paperless in their purchasing group has saved the company millions of dollars‚ so digitizing another manual paper process seemed like a sure bet. However‚ a recent change at top executive
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CASE STUDY CLOSING CASE: “PLANNING FOR THE CHEVY VOLT” 1. What does the Chevy Volt case tell you about the nature of strategic decision making at a large complex organization like GM? The Chevy Volt case tells me the nature of the strategic decision making at a large complex organization like GM can be difficult and challenging due to having to convince other managers to agree with the ideas for new strategies. In the case it states that the two persons‚ GM’s vice chairman and the head
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circumstance. As a traveler aboard the Mayflower‚ William Bradford was seeking religious freedom and sought someplace where he could practice his religion in peace. On the way over to the New World‚ his wife passed away. This was a major hardship for Bradford. He found strength in his faith because if he hadn’t stayed strong through his beliefs he would have lost control of himself. Death is something that is very tough to cope with and Bradford knew that seeking help in God to cope with his wife’s death
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INDIVIDUAL ASSIGNMENT Q1. What is meant by the term resources? What are the four factors of production and explain the factors incomes associated with each factor of production (20 marks) a) Resources means being able to produce something that can be a good or service. For e.g. the sun‚ trees‚ natural gas‚ materials‚ staff. b) The four factors of production are land‚ labor‚ capital and entrepreneurship. (i) Land- is considered the natural resources or raw materials we find on the earths surface
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A. Martin Manufacturing Company Historical and Industry Average ratios Ratio | Actual 2004 | Actual 2005 | Actual 2006 | Industry average 2006 | Current ratio | 1.7 | 1.8 | 2.5 | 1.5 | Quick ratio | 1.0 | 0.9 | 1.4 | 1.2 | Inventory turnover (times) | 5.2 | 5.0 | 5.3 | 10.2 | Average collection period | 50.7 days | 55.8 days | 58 days | 46 days | Total asset
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