Catalase Lab Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also‚ some cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism:
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Effects of Substrate Concentration‚ Reaction Time and Enzyme Concentration on Enzyme Reactions Corey von Ellm-St. Croix Rachael Kwan ID#: 20427841 Matthew Hrycyshyn & Saeideh Mayanloo Biol 130L‚ Section 017 Wednesday‚ 9:30am-12:20pm‚ 151 November 09‚ 2011 A living system controls its activity through enzymes. Enzymes are made from hundreds or even thousands of amino acids connected in a very unique and specific order. Almost all enzymes are proteins‚ except for ribozymes. The chain of amino
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bung. 7. Take measurement of gas after 2 minutes. 8. Repeat. Investigating the effect of enzyme concentration on the rate of an enzyme-catalysed reaction. James Moore Conclusion: For the conclusion we see that in fact the hypothesis is correct. When independent variable increases so does the dependent variable. As the amount of enzyme concentration increases so will the rate of the enzyme catalysed reaction. In the human body substrate is always available therefore there is only one
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Introduction: Enzymes are a substance produced by a living organism that acts as a catalyst to activate a specific reaction. The purpose of this experiment was to figure out if the temperature of the reaction would rise‚ will the absorption rise as well. Reactions use energy‚ If there is energy than heat occurs. The Hypothesis that was figured out was‚ If the temperature rises‚ then the absorption will also go up. The Independent variable that was tested was temperature. The dependent variable that
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the outcome of enzyme activity Introduction In this project I will monitor the rate of activity of Catalase. Catalase is an Enzyme which in the right conditions catalyses the decomposition of Hydrogen Peroxide into water and oxygen; 2H2O2 + Catalase >>> 2H2O + O2 Catalase is found in all cells and protects them from Hydrogen Peroxide which is a dangerous waste product that needs to be eliminated. Without Catalase living things could not survive. What are Enzymes? Enzymes are found in the
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and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient as the enzyme activity of the
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Lab #5: Refraction of Light Theory: Refraction can be defined as the bending of a wave when it enters a medium which causes it to have some reduced speed. In terms of light‚ refraction occurs when the ray passes through some medium which slows its speed; such as water or glass. In this instance the ray tends to bend towards the normal of the medium. The amount of bending or refraction which occurs can be calculated using Snell’s Law (). Objective: To measure the index of refraction of Lucite
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LAB 9: DATE: 25TH January‚ 2011. FORM CLASS: L6 3 SUBJECT: Biology TITLE: Enzymes AIM: To investigate the effect of temperature on the enzyme lipase INTRODUCTION: The phenomenon of catalysis makes possible biochemical reactions necessary for all life processes. Catalysis is defined as the acceleration of a chemical reaction by some substance which itself undergoes no permanent chemical change. The catalysts of biochemical reactions are enzymes and are responsible for bringing about almost
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What are amylases? Amylases are enzymes that break down starch or glycogen. Amylases are produced by a variety of living organisms‚ ranging from bacteria to plants and humans. Bacteria and fungi secrete amylases to the outside of their cells to carry out extracellular digestion. When they have broken down the insoluble starch‚ the soluble end products such as (glucose or maltose) are absorbed into their cells. Amylases are classified based on how they break down starch molecules i. α-amylase
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Omar Shbeeb Toothpick Enzyme Lab 9/25/13 Introduction Enzymes are used in all metabolic reactions to control the rate of reactions and decrease the amount of energy necessary for the reaction to take place. They are responsible for the thousands of chemical interconversions that sustain life. Enzymes are referred to highly selective catalysts‚ meaning they speed up the rate of metabolic reactions. To react‚ they need to find a perfect match with a substrate. They converge at a place called an
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