Taylor Hill Lab 6: Enzymes Purpose: The purpose of this lab is to test for enzyme activity‚ look at enzyme specificity‚ and how temperature affects enzyme activity. Hypothesis: I believe that the room temperature activity will produce the most activity. Materials: 3% hydrogen peroxide‚ a household blender‚ potatoes (3 medium size potatoes‚ that are peeled and quartered)‚ tap water‚ 8 test tubes‚ 8 transfer pipets‚ sugar packets‚ salt‚ a small saucepan‚ a refrigerator with a freezer‚ stove
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of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different enzyme can be found in any cell. Enzymes can be describe as molecules
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Toxicology Lab 1. In this investigation‚ a wide range of concentrations of Sodium Chloride (NaCl) solution were created and the effects that they had on radish seeds were tested. This ultimately created a doseresponse experiment in which it was detectable whether or not radish seeds were a reliable bioassay for the toxicity of NaCl. The goal of this experiment was to determine a correlation between toxicity and seed germination/radicle
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reaction. Name one microbe that would produce this response. * This change to the media will impart a blue color throughout the media or cause a blue band to form at atop of the media. This PH change is a result of proteolytic and degradative enzymes producing ammonia or amines form the breakdown of proteins. * Proteus
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The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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HB105: Introductory Physiology The Action of Enzymes Introduction An enzyme is located in all living cells‚ and is a complex protein molecules. These protein based molecule act as a catalyst. This is a compound that aids chemical reactions without its own structure and state being changed during the process. Catalysts speed up chemical reactions‚ changing substrates into specific produce. Without these enzymes life would not exist. Enzymes are fundamental to all living things as they speed up
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Enzyme Activity Lab Objective: To determine the effect of temperature‚ PH and enzyme concentration on catalase activity. Introduction: What is an enzyme and how the enzyme works. Any of a group of complex proteins or conjugated proteins thatare produced by living cells and act as catalysts in specific biochemical reactions. The system in which enzymes work is often referred to as the ’lock and key’ system it’s where the enzyme and the substrate lock together. The substrate then unlocks into
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To investigate the effect of temperature on an enzyme controlled reaction Introduction and planning For the investigation of enzymes‚ I am going to conduct an experiment to see how temperature can affect the rate of reaction of enzymes by testing it with starch. The enzyme that we are going to use is called amylase. We are going to test this enzyme with starch. By mixing amylase and starch solutions together under different temperature conditions‚ we can record the rate of reaction by taking a
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that the enzyme concentration will affect the rate of reaction but only up until a certain point because there is a limited supply of substrate. Enzymes are protein molecule that acts as biological catalyst by increasing the rate of reactions without changing the overall process. They are long chain amino acids bound together by peptide bonds. Enzymes are seen in all living cells and controlling the metabolic processes in which they converted nutrients into energy and new cells. Enzymes also help
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THE RATE OF ENZYME ACTIVITY. To investigate the effect that temperature has on enzyme activity I am going to use the enzyme amylase‚ which is used as a biological catalyst to break down starch‚ which cannot pass through the gut wall due to the size of the molecules‚ into smaller ones. Amylase is a carbohydrase‚ which converts starch to simple sugars in the Salivary Glands. Three features of all enzymes are: They are always proteins. They are specific in their actions and each enzyme controls one
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