Krupa Desai Cell Biology April 3‚ 2013 Lab: Biosynthesis of Starch Introduction: In this lab we learned the concept and procedure of synthesizing starch. We also learned the effects of pH and temperature on the reaction rates of amylase.. In the process of the synthesis lab we learned phosphorylation using a potato‚ which was what we synthesized. The phosphorylation took place after the addition of primer. There are two different types of starches used are amylose and amylopectin. To test
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Cheese lab Introduction Scientists work to create new and improved versions of cheese-curdling enzymes‚ as well as to improve the yields and qualities of cheeses. Modern-day cheese makers want to produce large amounts of high-quality cheese in the most economical way. Purpose ▪ Determine which curdling agent produces cheese the fastest. ▪ Determine which curdling agent produces the most cheese. ▪ Examine numerical data to support predictions. ▪ Examine variables that can
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Observing Enzyme Activity Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate. Hypothesis: If the enzyme is in a basic solution‚ then it will react faster because the enzyme (catalase) reacts better in basic solutions. Materials: 10 potato cubes (1 cm3) -Pipet Baking soda solution -50 ml glass beaker Bleach Water Lemon juice Vinegar 5 glass test tubes Drying rack Timer Graduated cylinder Hydrogen peroxide Procedure:
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Year 11 IB Biology IA Report 2011 5/31/2011 Enzyme Activity Experiment Introduction 3 AIM 3 Materials 3 Method 4 Result 5 Table 1: 5 Table 2: 5 Graph 1 6 Discussion 6 Conclusion 8 Bibliography 9 Appendix 1 (Test tube 3- 45 degrees) 9 Appendix 2 (Test tube 1- 5 degrees) 9 Appendix 3 (Test tube 2- 20 degrees) 9 Introduction Phenolphthalein is an indicator that is pink in alkaline solutions of about pH10. When the pH drops below pH 8.3 phenolphthalein will
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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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glucose. Even if oxygen is there or not‚ enzymes are mediated in the cytoplasm. The electron transport chain‚ chemiosmosis‚ and aerobic respiration use NADH molecule (which it main purpose is to transport electrons form one molecule to another) for later purposes. The mitochondrial matrix receives pyruvate from the cytoplasm after it crosses over the mitochondrial membrane. When the pyruvate enters the Krebs cycle it goes through many stages of biochemical enzyme-catalyzed reactions. In more detail
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withstand abnormal changes in pH without denaturing itself. Salivary Amylase is an enzyme that acts upon any polysaccharide that enters the mouth (mainly starch). However‚ it has a few other clinical uses. It is used for the diagnosis of acute pancreatitis‚ an inflammation of the pancreas‚ and other medical conditions regarding Amylase levels in the body. Amylase’s first discovery in 1831 led to new experiments about this enzyme. By separating pancreatic Amylase from Trypsin‚ they were able to realize that
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The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
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Introduction Chemical kinetics is the study of reaction rates. A reaction rate is the speed of the change in either reactants or products over a period of time. General kinetic rate equation is: Where [A] and [B] are the concentration of the species in the reaction. The variable k is the rate constant‚ which is a function of time and catalyst presence. The variables m and n are the order of reaction for their respective species concentration. The higher the value of the reaction order the
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In this lab we are going to be observing the decomposition of piglets over a month’s time. There are theory questions that have been given to us before and after the lab. We look back at our original theory to see where we went wrong‚ and then correct it. The lab was disgusting‚ surprising‚ and very interesting. The first questioned to be answered is which piglet decomposes faster‚ a piglet that is in its natural state‚ that is burnt‚ that is buried‚ and that is buried in a wooden box? With
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