baking Yeast helps raise the dough and also yeast is used in the process of making wine.The Reason why yeast was chosen to was to see how yeast can metabolize different sugars and how much Co2 they release when when metabolizing.To test the yeast at the lab we used 4 different types of carbohydrates which are known as Glucose‚Lactose‚Sucrose‚and Starch.The main thing that make some of these carbohydrates different from each other is if it’s a Monosaccharide‚ Disaccharide‚or Polysaccharide. Monosaccharide
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Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10‚ 37‚ 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start
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Biochemistry is the chemistry of biological systems. The practical component of biochemistry is aimed at developing your interest in and understanding modern biochemical and molecular biological experimentation. The techniques learnt in the biochemistry lab will be applicable to all life sciences. THE OBJECTIVES OF THE BIOCHEMISTRY LABORATORY INCLUDE: (1) Learning the theory behind the techniques and biochemical pathways (2) Learning the physical skills and techniques of modern experimental biochemistry
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firstly amplified by PCR‚ then cloned in the pBluescript plasmid vector. EZH2 is an enzyme which adds methyl groups to the twenty seventh amino acid (Lysine) and is encoded by EZH2‚ the EZH2 gene encodes part of the Polycomb group which make protein complexes that help to maintain genes transcriptional repressive state over successive cell generations. http://www.ncbi.nlm.nih.gov/gene/2146 Throughout this lab report template DNA that contains the gene EZH2 was provided‚ this will be amplified
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Therefore this supports my hypothesis: At pH buffer 7 the enzyme (catalase) will work most efficiently because at pH levels too high or too low will cause the enzyme to denature‚ because they alter the charged groups of amino acids that make up the active site causing certain ions to be attracted and cluster around the negatively charged group‚ therefore interfering with the binding of substrate and changing the rate of activity of the enzyme. This is‚ because the ions that have clustered around the
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Research Question How will the addition of different pH buffers to amylase affect the rate of starch digestion measured using starch and iodine? Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1‚ 4‚ 7‚ 10‚ and 14) on the function of amylase by using starch and iodine. Usually
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(Justification-2 Enzyme Inhibition) By quantitative balance‚ the total amount of Enzyme is [E] 0= [E] + [EI] + [ES] + [ESI]. By using a=1+[I]/KI and a′=1+[I]/K′I‚ it is followed by [E]0=[E]a+[ES]a′ This equation can be written like this‚ [E]0=(Km[ES])/([S]0)a + [ES]a′=[ES]( aKm/[S}0+a’)‚ because of Km=[E][S]/[ES] and [S]≈[S]0. V=kb [ES] =kb [E] 0/ (aKm/[s] 0+a’). Kb [E] 0 is Vmax. This is why V=Vmax/(a^’+aKm/[S]0). This equation can be rearranged like this‚ 1/V= a’/Vmax+(aKm/Vmax)1/[S]0‚ which is
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MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________ EXPERIMENT 1 TITLE: Observing Bacteria and Blood OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood. PROCEDURES: Using the microscope‚ an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood: After reviewing the section of the manual
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Potato Catalysis Lab Pre-Lab Assignment Background Information: An enzyme is catalytic protein. It is the most important type of molecule found in living cells. Cells would not be able to function without enzymes. Enzymes speed up or slow down chemical reactions of the cells. It is usually easy to identify the names of enzymes because they end in -ase. The enzyme that acts upon the substrate hydrogen peroxide is usually called catalase. This enzyme is found in both plants and animals
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Tittle : Investigation of the Enzymatic Effects of Materials on Hydrogen Peroxide Solution Objective: To investigates the enzymatic effect of various materials in the hydrogen peroxide solution. Table 1 Test Tube Contents with 5 cm3 hydrogen peroxide Observations before and after using wood splint Observation of after Observation of after adding hydrogen using wooden glowing peroxide splinter 1 Fresh liver Moderate
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