point for the enzyme (catalase) to substrate (H2O2(aq)) concentration ratio. Thus‚ to truly understand this‚ the trial time period should be extended insofar that a declination in the rate of the reaction can be observed with multiple trials. If the trends of the independent trials coincide with one another‚ then it is plausible that a saturation point may have been a factor of the linear-like trend. This case will be further discussed in one of the five major factors that influence enzyme activity:
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being digested with EcoRI restriction endonucleasse. Procedures: λ DNA and puC18 DNA were put into two tubes respectively. Then‚ EcoRI buffer‚ EcoRI enzyme and deionized water would be put into both tubes. EcoRI enzyme was the restriction enzyme that cut the DNA at the specific sequence. The EcoRI buffer enhanced the stability of many enzymes and binds contaminants that may be present in DNA preparations. DI water was used to bring the solution into a required volume for gel electrophoresis.
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In the first part of the lab‚ we prepared a salivary amylase solution and aggregated acetic acid to detect the presence of mucin. Then‚ 4 test tubes were made of the following: Tube 1 = 3 ml starch + water + 37 degrees water bath; Tube 2 = 3 ml starch + saliva in water bath; Tube
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I also hypnotized that the enzyme is acidic on the pH scale because it exists in our small intestine and our small intestine uses acid to break down food. The result from the lab prove my hypothesis correct because lactase worked well at body temperature and it also worked well at pH of 4.5 to 5.5 mg/dl proving that lactase works best at an acidic pH number. The lab shows that the highest amount of glucose is made by temperatures close to body temperature. In our our lab‚ we found that at 35 °C 500
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ABSTRACT: This lab allows us to observe the conversion of hydrogen peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this decomposition reaction. The catalase enzyme acts as catalysis‚ helping lower the energy needed to activate the reaction while the enzyme itself is not affected. Catalase is a digestive enzyme used to break down hydrogen peroxide‚ which is a normal byproduct of cellular respiration. The reaction could take place without the help of catalase‚ but
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amylase lab‚ it was being tested if amylase‚ an enzyme found in saliva‚ would be denatured by being put in an acid or high temperatures. This lab is about denaturing amylase. It is tested by exposing it to pH and temperature changes. It is then mixed with Benedict’s solution‚ is a solution that changes color from blue to reddish brown when maltose is present. Amylase breaks starch into maltose‚ so is the amylase isn’t denatured‚ it should change colors. Amylase is an enzyme. Enzymes are a type
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system Tongue • For taste • Pushing food into the esophagus Papillae • Rough edges of that tongue that contain the taste buds Hard and soft palate • Separate the nasopharynx from the mouth Parotid gland • Secretes saliva (amylase enzyme) which breaks down starches into sugar Stomach • Stores food • Begins digestion of tissues and proteins by secretion of gastric juices. These juices are very acidic (pH 1-2). The semi-digested stomach contents are called chyme Esophagus
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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How Enzymes work in the home and in industry essay This essay aims to explore the ways on how enzymes are used in home and in industry‚ and it aims to explain the advantages and disadvantages of using enzymes in the home and industry. An enzyme is a protein that is formed by the body that acts as a catalyst to cause a certain desired reaction. Enzymes are very specific. Each enzyme is designed to initiate a specific response with a specific result. Firstly‚ the AQA Science Biology textbook published
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can affect the optimum operation of enzymes. These condition include temperature‚ enzyme concentration‚ substrate concentration‚ acidity‚ salinity‚ and any present activators/inhibitors. In this particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes; The enzyme catalase has an optimum operation
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