How Enzymes Work In Different Environments By Sarah Smith Biology1111 October 20‚ 2011 Lab Partner: Nellie Greer ABSTRACT Peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide‚ H2O2‚ into O2 gas and water. We examined the different pH environments that can affect the enzyme activity during the breakdown of H2O2. In order to do this‚ we added different levels of pH‚ low‚ medium‚ and high‚ into different test tubes with the enzyme and H2O2‚
Premium Enzyme PH Chemical reaction
Lab #4: The Immune System Purpose: The purpose of this lab was to perform and understand the procedures of conducting an ELISA test to determine whether a particular antibody is present in a patient’s blood sample through a virtual simulation. Hypothesis: If I successfully complete this lab‚ I will then understand how to perform an ELISA test‚ the purpose an ELISA test‚ and also how to interpret the results of this test. Materials and Procedures: Materials: Howard Huges medical
Premium Antibody Immune system Immunology
Enzyme Controlled Reactions 1) Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases? A) The relationship between the substrate concentration and the initial reaction of an enzyme-catalyzed reaction is very productive‚ but is dramatically affected by the pH level of the given solution. The most productive pH level is
Premium Enzyme PH
Lab Report An enzyme is a protein that speeds up the rates of chemical reactions. They recognize‚ bind‚ and change specific reactants. They do not change so they can catalyze the same reaction again and again. Activation energy is the amount of energy needed in order to begin a chemical reaction. A Catalyst is a substance that increases the rate of a chemical reaction without itself undergoing any permanent chemical change. Catalysts are substances or a substance that configures another substance
Premium Chemical reaction Chemistry Catalysis
Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
Premium Glycerol Glucose Liver
Introduction: During this unknown lab report various test were performed to differentiate microbes from each other and to compare metabolic and biochemical process. The gram stain distinguishes between Gram positive and Gram negative bacteria based on the composition of the cell wall. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan
Premium Bacteria Adenosine triphosphate
The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
Premium Antibody Immune system ELISA
Julie Kim Free Fall Lab Purpose: to use collected data and the kinematics equations to determine the value of local gravity Data: height 161 cm(1 m/100 cm) = 1.61 m mass of small ball 16.5 g mass of big ball 28.0 g 1 2 3 4 5 6 7 8 9 10 Average Small 0.585 sec 0.571 sec 0.567 sec 0.571 sec 0.571 sec 0.572 sec 0.571 sec 0.574 sec 0.576 sec 0.571 sec 0.573 sec Big 0.573 sec 0.568 sec 0.569 sec 0.569 sec 0.570 sec 0.569 sec 0.571 sec 0.563 sec 0.571 sec 0.570
Premium Gravitation Acceleration General relativity
Free Fall Lab Natalie Soria Lab Partners: Ryan Michaely Iqra Haji Yan Huang 1. Purpose: The purpose of this experiment is to determine the acceleration due to gravity by observing the motion of a free falling object. 2. Equipment Used: A. Timer Switch B. Time-of-Flight Accessory C. Control Box D. AC adapter E. Drop Box F. Steel ball G. Solid gold ball H. Big plastic ball 3. Method Used: 1) Place the steel ball on the
Premium Classical mechanics Force Mass
Enzyme Catalysis Maltose sugar is broken apart by maltase enzyme Substrate are molecules enclosed in the enzyme Catalase: found in every living thing Takes two molecules of hydrogen peroxide and converts it irreversibly to create oxygen gas and water 2H2O2O2+2H2O Question: What variable affects the rate of enzyme catalysis most? Variables Tested: Hydrogen Peroxide concentration‚ yeast concentration‚ heat and pH Materials: 10% glucose mixture 1.5 %‚ 3% and 6% peroxide mixture Yeast
Premium Enzyme Oxygen Hydrogen peroxide