firstly amplified by PCR‚ then cloned in the pBluescript plasmid vector. EZH2 is an enzyme which adds methyl groups to the twenty seventh amino acid (Lysine) and is encoded by EZH2‚ the EZH2 gene encodes part of the Polycomb group which make protein complexes that help to maintain genes transcriptional repressive state over successive cell generations. http://www.ncbi.nlm.nih.gov/gene/2146 Throughout this lab report template DNA that contains the gene EZH2 was provided‚ this will be amplified by
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Lab 3 : Techniques in Microscopy Title : Preparation of light microscope observe cell organisms. Objective : To observe and investigate the cell structure under the light microscope. Introduction A microscope is an instrument used to magnify and resolve a specimen. It is important to know several important features of microscopy which involves magnification‚ resolution and contrast. Magnification is the enlargement of a specimen while resolution is the ability to distinguish detail or the
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CE121: Construction Materials Laboratory Report No. 1 Particle Size Distribution Van Joseph E. Casalme 2010-14928‚ BS Civil Engineering Institute of Civil Engineering‚ College of Engineering University of the Philippines Diliman‚ Diliman Quezon City 1101 Submitted to: Engr. Christian R. Orozco ABSTRACT This test (ASTM C136-06) determines the grading of materials being used as aggregates using two parameters (coefficient of uniformity and gradation) from particle-size distribution
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Solute Concentration of Potatoes Lab #1 Purpose: To find the molarity/concentration of potato cytoplasm. Materials: As on page 1 in the lab handout. Procedure: As on page 1 in the lab handout. Data and Observations: Test Tube # | Concentration of sucrose solution (mol/L) | Initial Mass (g) | Final Mass | Percentage change in mass | 1 | 1.0 mol/L | 3.00g | 2.25g | -25.0% | 2 | 0.9 mol/L | 2.70g | 2.07g | -23.3% | 3 | 0.8 mol/L | 2.92g | 2.25g | -22.9% | 4 | 0.7 mol/L | 2.60g
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Acid Base Titration Purpose: The purpose is to calculate the molarity of a NaOH solution by titrating the base with 5mL of standard HCl solution in each trial. By adding the base with unknown molarity to the acid with 0.10M the molarity of NaOH can be calculated. The base‚ NaOH‚ helps bring the pH of the acid‚ HCl‚ closer to seven‚ which neutralizes it. When using the buret the amount of NaOH used is able to be determined. Then by writing a balanced chemical equation and using the titration
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a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular reactions. All proteins are made up of amino acids (Johnson‚ 2010). The purpose of this experiment is to determine whether peptide bonds are present in various food substances. The color violet will indicate the presence of
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Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
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simple mechanisms used to convert rotary motion into oscillating linear motion and vice-versa. The first of these is the slider-crank - a mechanism widely used in engines to convert the linear thrust of the pistons into useful rotary motion. In this lab we will measure the acceleration of the piston of a lawn mower engine at various speeds. The results exemplify a simple relation between speed and acceleration for kinematically restricted motions‚ which will discover. An adjustable slider-crank apparatus
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Free trade: For or against? Muntianu Ioana Hassan Chaudhry Anna Dvurechenskaya Guliza Toran 2/18/2013 Ioana For centuries economists have sustained free trade as the best free trade policy. Even so‚ after a more in depth analysis one can notice certain advantages and disadvantages of free trade. This report explains the concept of free trade‚ the implications of engaging in free trade and the pro and con opinions along with an explication of certain theories related to the concept.
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Enzyme Lab Name ___________________________ Assignment 1: Getting to Know Enzyme Lab: Setting Up an Experiment The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments. Click on each item in the lab to learn more about its purpose. Once you are familiar with the lab‚ click on the Experiment button to begin the first assignment. This assignment is designed to help you become
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