plasmids such as the TOL and OCT plasmid. Besides that‚ it has a circular genome where around eighty genes in oxidative reductase can be found. All these three components found in Pseudomonas putida are important in bioremediation such as degrade toluene and in the application to biotechnology. Pseudomonas putida plays an important role in degradation of pollutants in environment. It has the ability to produce Poly-3-hydroxyalkanoates (PHA) from aromatic hydrocarbon styrene‚ which is a major environment
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concentration and after concentrating all fractions of these concentrations‚ approximately 7 to 8 mg of quercetin was obtained. 4.2 Thin layer chromatography TLC profiles were carried out for the fractions‚ performed using the solvent mixture of toluene: ethyl acetate: methanol in the ratio of 5:3:2 (v/v/v) and this mobile phase enabled excellent separation of the bioactive compound. The results showed that there was good amount of correlation between the standard quercetin band and the band of bioactive
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oil bath after 45 minutes. 5. Pour the reaction mixture immediately after removal from the oil bath‚ into beakers containing 50 mL stirred methanol. A white precipitate should settle out. If the product is difficult to pour‚ add a small amount of toluene (2 mL) to dissolve it and then pour into methanol. 6. Continue to stir in methanol for another 10-20 minutes. 7. Vacuum filter the solid polymer and wash with methanol several times. 8. Air-dry the polymer or dry the polymer in a vacuum oven at room
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Assignment 10: Environmental Health Aaron CottonBetteridge (10576005) Go to Air Pollution: List 5 gases found on this link ● ● ● ● ● ● Benzene Perchloroethylene Methylene chloride Dioxin Asbestos Toluene Go to Acid Rain: What are the two major gases that are responsible for Acid Rain? Sulfur dioxide (SO ) and nitrogen oxides (NO ) are the two major gases that are responsible for 2 x Acid Rain. Go to Lead: What are 4 health effects listed as consequences of Lead Poisoning?
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would be miscible or immiscible. It may be helpful to look up some of the chemical structures. (3pts) a. Benzene and water b. Water and methyl alcohol c. Hexane and benzene d. Methylene chloride and benzene e. Water and toluene f. Ethyl alcohol and isopropyl alcohol 3. Consider a crystallization of sulfanilamide in which 10mL of hot 95% ethyl alcohol is added to 0.10g of impure sulfanilamide. After the solid has dissolved‚ the solution is cooled to room
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anthrone in 100 ml of ice-cold 95% H2SO4. Prepare fresh before use. Standard glucose: Stock —Dissolve 100 mg in 100 ml water. Working standard— 10 ml of stock diluted to 100 ml with distilled water. Store refrigerated after adding a few drops of toluene. PROCEDURE 1- Weigh 100 mg of the sample into a boiling tube. 2- Hydrolyse by keeping it in a boiling water bath for three hours with 5 mL of 2.5 N HCl and cool to room temperature. 3- Neutralise it with solid sodium carbonate until the effervescence
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Arenes The aromatic hydrocarbons also have the name arenes. They contain in their molecule one or more cycles made up from 6 carbon atoms. When the molecule is formed out of a single cycle‚ the hydrocarbons are mono nucleuses; when the molecule contains more than one cycle‚ the hydrocarbons are poly nucleuses. The simplest aromatic hydrocarbon‚ benzene‚ is compound out of just one such cycle; its formula is C6H6. The representation of benzene through a cycle of 6 carbon atoms with 3 double bounds
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types of blood cells: red cells‚ white cells‚ and platelets (pancytopenia) Causes A-Primary: 1- congenital (as Fanconi’s anemia). 2- Idiopathic acquired (67% of cases). Causes B- secondary: 1-chemicals (benzene‚ toluene‚ glue sniffing). 2-drugs (chemotherapeutics‚ antibiotics‚ gold‚ phenytoin). 3-insecticides. 4-ionizing radiation. 5-infections (as hepatitis). 6-paraxysmal nocturnal haemoglobinuria. Pathogenesis Reduction
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BIOREMEDIATION INTRODUCTION:- The process of bioremediation was reportedly devised by George M Robinson in the 1960 during experimentation with dirty jars and the introduction of microbes this was however not applied until the 1972 clean out of the fuel holding tanks on the RMS queen mary. Science 1972 bioremediation has become greater developed as a means of cleaning up spills of gasoline diesel ‚ heavy metals and other easily degraded petroleum products (National Research Council 1993). Bioremediation
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it be? Will they grow? Will the die? What is it going to happen? RESEARCH Gasoline is obtained from petroleum in oil refineries‚ and used as fuel in combustion engines. It is made of over 250 types of hydrocarbons. Aromatic hydrocarbons like toluene and benzene are used to increase the octane rating of gasoline. Other additives are also added to improve engine performance and reduce the emission of harmful gasses formed as byproducts of the combustion process. Some hydrocarbons found in gasoline
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