Human Embryonic Kidney(HEK) 293 cells were transiently transfected with pEGFP-N1(Clontech‚ Palo Alto‚ CA‚ encoding enhanced GFP as an indicator of transfection) and pEGFP-LeIF plasmids cloned at previous studies(Ghafarifar and et al‚ in press) by Calcium phosphate and non- tranfected cells was used as negative control. briefly‚ Hek-293 cells were grown at 37°C in Dulbecco’s Modified Eagle Medium (DMEM‚ Gibco) supplemented with 10% Fetal Bovine Serum (FBS‚ Gibco) and 5% CO2. Log-phase cells were employed
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High-Low Method The controller of PATAY Hospital would like to come up with a cost formula that links admitting department cost to the number of patients admitted during a month. The admitting department’s costs and the number of patients admitted during the past nine months follow: Month Number of Patients Admitted Admitting Department’s Cost April 18 P 15‚ 600 May 19 P 15‚ 200 June 17 P 13‚ 700 July 15 P 14‚ 600 August 15 P 14‚ 300 September 11 P 13‚ 200 October 11 P 12‚ 800 November
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The BSA‚ or Bank Secrecy Act is comprised of six parts; The Patriot Act‚ MITR‚ CTR‚ MIP‚ SAR‚ and OFAC. The use of all of these in tandem makes it easy to catch fraud‚ money laundering‚ Terrorist funding‚ Criminal organizations and more. The six parts each play their parts in this‚ with some being smaller and less complicated but no less important. The USA PATRIOT act is a big one‚ and very broad. However this was on purpose so that way it can encompass all types of transactions with very few loopholes
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APPROVAL LETTER September 03‚ 2010 Dr. Savino Juan A. Nagtalon Vice President for Academic Affairs Pilgrim Christian College Dear Sir‚ Good Day! The undersigned English 12 students of Pilgrim Christian College are currently conducting a research study entitled “COMPUTER ADDICTION”‚ in partial fulfillment of the course. In this connection‚ we would like to ask your permission to allow us to conduct the study here in Pilgrim Christian College to selected college and high school
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* Necessity for Close Supervision The necessity for a considerable amount of supervision on the part of the credit manager over his staff is quite apparent. Credit men operate during much of the working time away from the home office removed from definite and direct executive control. However‚ one method which could make the credit men do their job‚ as expected of them is to give them a “Deadline” for the case they have under investigation and study. Psychologists suggest three important appeals
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A protein standard curve was generated by using the protein standard (1 ampule) measurement at 595 nm. Standard curve was prepared as shown in Table 4. Bovine serum albumin (BSA) was diluted with distilled water. The absorbance of each sample were measured using spectrophotometer at 595nm. Standard protein curve was plotted according to the concentration of protein where the x-axis and y-axis represent protein standard concentration and absorbance (595 nm) respectively. 3.10.2 Protein Determination
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are teaching strategies‚ passing rate‚ faculty‚ handouts and materials‚ and environment conducive to learning. Implications for the review schools and recommendations are discussed. Students who are taking up Bachelor of Science in Accountancy (BSA) are expected to be a Certified Public Accountant (CPA) someday. In order to be a CPA they need to pass the licensure examination for accountants‚ where they would need obtain a general average of seventy-five percent (75%)‚ with no grades lower than
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Frequent Shopper Program‚ Part I Zeferino Reyes BSA - 385 October 31‚ 2011 Joseph Aguirre Frequent Shopper Program‚ Part I Smith Systems Consulting has been contracted to develop a Frequent shopping program for the business of Kudler Fine Foods. Smith Systems Consulting was founded in the year of 1984‚ with only five individuals and has increased in size to become a massive consulting firm‚ thus being requested to help Kudler Fine Foods‚ (Apollo Group‚ 2003‚ 2004). Kudler Fine Foods
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Each student carriers with them‚ a series of life experiences that have helped them grow in different aspects of their lives. As a student who has encountered many different cultural‚ economic‚ and academic environments‚ I believe that in order to reach our fullest potential we must try to learn from the experiences of each student. I believe that the most successful student is one who is motivated and persistent in their studies‚ has knowledge of the world around them‚ and manages their time
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Case Study: What will you do? Your organization is undergoing human resource reengineering and it was agreed by your Management Committee (ManCom) to terminate the services of the “deadwoods” (those unproductive and obsolete employees) of the organization. In reviewing their employment records‚ you found out that the “deadwoods” involved have been in the company from 12 to 17 years and they practically gave the best of their working lives to your company. Per year company policy‚ full retirement
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