curve with the understanding that the results on the unknown protein are semi-quantitative. Since most proteins are not available in large quantities‚ standard curves for protein assays are typically based on the use of either bovine serum albumin (BSA) or bovine gamma globulin (IgG). The Bradford protein assay is a method to determine protein concentration in the mixture. This method is based on the proportional binding of the dye Coomassie to proteins. If there is a higher protein content‚ the
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concentration being determined. Objectives 1. To prepare a standard curve of absorbance versus protein concentration by using Bovine Serum Albumin (BSA). 2. To determine BSA concentration in two sample solutions. 3. To determine protein concentration in apple juice. Procedure Part1 7 test tubes were prepared and labeled. The appropriate amount of BSA and distilled water were pipetted into the respective test tubes according Table 3.1. 500µl of Branford Reagent were added into all the test tubes
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Course Syllabus BSA/310 Version 7 1 Course Syllabus College of Information Systems & Technology BSA/310 Version 7 Business Systems Course Description This course reviews common business systems and their interrelationships. Business systems covered include finance‚ accounting‚ sales‚ marketing‚ human resources‚ legal‚ and operations. Emphasis is placed upon the inputs and outputs of information systems‚ the potential for integration of the systems‚ and information systems security. Policies
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and remained the same in about 35%. However‚ the worldwide piracy rate went up from 38% in 2007 to 41% in 2008. The global rate rose for the second year in a row because PC sales grew fastest in high-piracy rate countries such as China and India. (BSA‚ IDC‚ 2009) Among the lowest piracy countries‚ Taiwan has a higher piracy rate of 39% and loss of $201 million dollars. Ethics balances the social moral value and order with its invisible but powerful existence‚ and it is the guideline of behaviors
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1. Describe the mechanism by which green fluorescent protein (GFP) is able to emit fluorescent light and why ultraviolet light is required to visualise it. (5 marks) When GFP is hit with UV light‚ the chromophore is hit by a photon. This changes the chromophore from ground state (A) to A*‚ which is a highly excitable state. Due to such a highly excitable state not being able to remain so for very long‚ the A* state chromophore emits a proton‚ lowering its state to I*‚ the energetic I. This I* state
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which are the promotion of an electron from an occupied orbital to an unoccupied orbital (Pavia et.al 2009). In this investigation‚ the aim is to determine unknown protein concentrations from the Bovine Serum Albumin (BSA) standard curve and observe how the protein concentration of BSA and Lysozyme is affected by the addition of detergents‚ Sodium Dodecyl Sulphate (SDS) and Triton X-100. This study involves two procedures. Firstly a protein assay needs to be carried out in order to investigate the
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Expression and Purification of Human Platelet-Derived Growth Factor Beta (PDGF-B) Rachel C. Hermina-Stewart* and Elsie I. Parés-Matos† *Industrial Biotechnology Program‚ †Department of Chemistry University of Puerto Rico-Mayagüez Campus rachel.hermina@upr.edu December 14‚ 2009 ABSTRACT. Platelet-Derived Growth Factor (PDGF) is one of many proteins that regulate cellular growth and division. The PDGF family consists mainly of five different isoforms called as PDGF-A‚ PDGF-B‚ PDGF-C and PDGF-D. Three
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Title: Purification of Egg white protein Name: Michael Johnson Partner: David Logad & Nandita Date: 2nd 9th September 2004 Group: Thursday 11:30am - 3:30pm Introduction Salting Out In 1888 Hofmeister that it can be possible to dehydrate a protein by adding salt to the solution‚ salting out. When a protein in a aqueous solution it is surrounded by water‚ in fact there can be up to 0.35g of water tightly bound to 1g of protein (Simpson 2004). Also the effectiveness of the salting out
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BSA Corporation Limited ( Earlier BSA Facilities Limited ) Wiring harness Division www.bsagroup.in December 8‚ 2011 Company profile BSA Facilities Ltd. is a fast growing diversified professional organization established in the year 1995 outsourcing various activities related to human resources based on the skills requirement of customers. BSA Wiring harness is formed after Acquisition of Tyco Electronics Wiring harness Business at PAN India Level. BSA acquired the Plant in July-2009. BSA
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blocking time was investigated. Different time periods were examined and it was narrowed down to 15minutes as the sufficient blocking time. Smaller time intervals were studied to further shorten the blocking time. As shown in Figure 4(a)‚ no BSA blocking resulted in strong nonspecific binding of the QD-mAb probe on liver’s surface both dorsal and ventral sides. As the blocking time increased‚ nonspecific binding decreased and reached the saturated point at 10 minutes. The integrated intensity
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