of protein concentration of an unknown sample of BSA‚ and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of the unknown solution of BSA together with other known concentrations‚ and then
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595nm. Using the BSA stock solution (2mg/mL=2000 ug/mL)‚ we prepared the concentration of the protein standard by carrying out serial dilution at 2x‚ 5x‚ 10x‚ 20x and 40x. The 2x dilution was made by mixing 250 uL of BSA and 250 of the homogenizing buffer. The solution was then vortexed for 10 seconds. A similar procedure with different concentration of BSA and stock solution was carried out to find the other diluted concentrations. After the dilutions were made‚ 200 uL of the BSA concentration from
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“I don’t know if all Boy Scouts are gays‚ they could probably tie the knot in‚ like‚ fifty different ways.” Bo Burnham begins his song I’m Bo‚ Yo with those lyrics‚ and while it may be fun to make jokes about the Boy Scouts of America (BSA)‚ their short shorts‚ wacky handshakes‚ and neckerchiefs all being prime targets‚ the issue of homosexuality in the organization has always been a serious one. In fact it’s such a big issue it has its own Wikipedia page. And it’s old‚ going back to the organization’s
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Cited: “About BSA.” BSA | The Software Alliance. BSA‚ 2014. Web. 7 Mar. 2014. Anthony‚ Sebastian. “A Case for Piracy.” PC Magazine (2013): 35-38. MasterFILE Premier. Web. 6 Mar. 2014 Bilton‚ Nick “Enforcement.” BSA | The Software Alliance. BSA‚ 2014. Web. 20 Mar. 2014. Espinel‚ Victoria‚ Aneesh Chopra and Howard Schmidt. “Combating Online Piracy while Protecting an Open and Innovative
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and remained the same in about 35%. However‚ the worldwide piracy rate went up from 38% in 2007 to 41% in 2008. The global rate rose for the second year in a row because PC sales grew fastest in high-piracy rate countries such as China and India. (BSA‚ IDC‚ 2009) Among the lowest piracy countries‚ Taiwan has a higher piracy rate of 39% and loss of $201 million dollars. Ethics balances the social moral value and order with its invisible but powerful existence‚ and it is the guideline of behaviors
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solution particles are absorbed‚ and this happens at a specific wavelength. Since the Beer Lambert law is useful only for a range of wavelengths‚ it is not applicable to all protein solutions. In this experiment‚ an absolute standard was calculated using BSA‚ so that the concentrations of the other unknown protein solutions can be determined (Lambert et.al‚ 2011).The different assays used for this protein quantification were Lowry‚ Bradford (Coomassie Blue) and UV direct. Protein assays help to determine
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Everybody grows up for a reason; a reason that parents would consider as blessings. Many parents would consider their children as their heirs and the ones who will work and take care of them when the time comes that they retire in their respective jobs as they grow old. They expect their children to be successful and have a good career condition. Career is a goal that came out from a long time desire and ambition which it is not affected by time or money. A career that yields great income does not
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Effects of Facebook Addiction in the Study Habits of 2nd Year BSA Students of GenSantos Foundation College Incorporated In Partial Fulfillment of Requirements in Technical Writing Members: Averilla‚ Angelie Baisac‚ Julie Ann Estrebilla‚ Reah Giducos‚ Richie Subang‚ Kristia Lou March 2011 ACKNOWLEDGEMENT As researchers‚ we would like to extend our debt and gratitude to the following person who has been part of completing this requirement. We would like to direct
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purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally Affinity Chromatography on a Cibacron blue Sepharose stationary phase. Using BSA‚ which is analogous for BCA assays‚ a standardization was created to understand where the protein concentration was for each fraction. Introduction: This experiment is a continuation of the lab’s efforts to purify L-LDH. Previously our enzyme was purified
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unproven inferences to circulate around. For the following inception of school days‚ it has been evident that Bachelor of Science in Accountancy (BSA) freshmen enrollees has largely grown in population. Compared to the other related courses under Accountancy Department such as the Bachelor of Science in Management Accounting and Accounting Technology‚ BSA comes out to have __ in number for the school year 2012-2013 wherein BSMA and BSAT have __ and __‚ respectively. This secures related and cached
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