temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature up to 50oC speeds up the rate of enzyme activity and any increase in temperature after 50oC reduces rate of enzyme activity drastically. Results
Premium Enzyme Energy Kinetic energy
O2 +H2O → H2O2 + gluconate aminophenazone + phenol + H2O2 → a red dye + H2O2 Under stable conditions‚ absorbance measured using a spectrophotometer will be proportional to the amount of glucose present (schedule Coventry‚2013). Spectrophotometry is a method used to measure absorbance of light. This measurement is carried out using a spectrophotometer. A spectrophotometer is an equipment used to take accurate measurement of absorbance at various wavelengths. Electrons are usually present at different
Premium Glucose Sugar Fructose
Boy Scouts of America (BSA) Boy Scouts of America is one of the biggest youth organizations in the United States. The mission of the Boy Scouts of America is to prepare young people to make ethical and moral choices over their lifetimes by instilling in them the values of the Scout Oath and Scout Law. The Scout Oath reads‚ “On my honor I will do my best to do my duty to God and my country and to obey the Scout Law; to help other people at all times; to keep myself physically strong‚ mentally
Premium
Experiment 2: Absorbance and Spectrophotometry ABSTRACT: This was an investigation into the effects of different wavelengths of light on methylene blue and carmine red on the absorbance value on a spectrophotometer. A spectrophotometer is used to measure light intensity by emitting a single light source through a cuvette of coloured solution. The particles in the solution‚ which are coloured‚ absorb the light depending on how concentrated it is and this produces an electronic reading from
Premium Light Color Red
disease in which there are high levels of sugar in the blood (Diabetes). The spectrophotometer was applied to this lab to determine the absorbance of blood glucose in diabetic and non-diabetic blood samples. In order to prove this‚ tests were conducted by taking the blood samples at different times right before a meal was eaten then 30‚ 60‚ 90‚ and 120 minutes after the meal. The 6 test tubes had been placed in the spectrophotometer to measure the absorbance of blood glucose in the diabetic and non-diabetic
Premium Insulin Diabetes mellitus Blood sugar
Chapter 1 The Problem and its Background Introduction Study habit is defined as the regular tendencies and practices that one depicts during the process of gaining information through learning. In short‚ it is the way that you study. It includes time management or how much time you afford in studying‚ your desk‚ the lightings‚ what subject you start on studying‚ whether you study with or without music‚ do you take down notes or not‚ do you sit or lie down‚ etc. Despite the fast pacing growth
Premium Effect Cagayan de Oro City Learning
distance‚ followed by chlorophyll a. Chlorophyll b is the most polar; therefore‚ it travels the shortest distance. The separated pigments on the chromatography paper can be eluted in acetone and absorbance spectrum is determined using spectrophotometer. Spectrophotometer produces a graph of the absorbance spectrum which shows the wavelengths (in nm) that are absorbed by the pigment and that are reflected. The reflected wavelength of chlorophylls a and b reflect the wavelengths of l light that are associated
Premium Color Chemical polarity Chromatography
spectrophotometric analysis. Acidified solutions (using 0.1 M HCl) of FeCl3 and KSCN were prepared for the calibration of the UV-Vis spectrophotometer (using standard solutions) and the determination of Keq of the Fe(SCN)2+ formation (solutions with unknown concentrations). Absorbance readings of the standard and unknown solutions were obtained through the spectrophotometer. The equation of the trend line of the calibration curve (obtained through the plot of the absorbance readings vs. thiocyanatoiron
Premium Chemical equilibrium Concentration
produced. With help from the stain reagent benzoic acid – I visibly saw the production of sugar molecules. However‚ I was only able to determine which of the Tubes experimented had the most or least sugar present‚ by color intensity. By operating a spectrophotometer‚ I was able to create a standard curve‚ which gave the absorbance values of glucose/maltose concentrations in the Tubes tested. From that‚ using the standard curve results and calculations (7.8 x 10^16) I then determined the µmoles of sugar present
Premium Enzyme Glucose Starch
CCN2231 – GENERAL BIOCHEMISTRY Lecturer: Dr. IVAN CHUNG GROUP PROJECT LAB REPORT 1 Group B Student Name: Yip Ho Yin (Student ID:12309985A) Student Name: Wong Kin Hin (Student ID:12310084A) Student Name: Ling Tung Yin (Student ID:12307848A) Student Name: Cheuk Hiu Fung (Student ID:12309957A) Student Name: Wong Hoi Tung (Student ID:12309501A) Date of submission: Date of Experiment: Introduction
Premium Liver Glucose Red blood cell