in the sample and can be measured at 550nm. Materials : 1. Total bilirubin reagent. 2. Direct bilirubin reagent. 3. Calibrator. 4. Sample X. 5. Appropriate number of tube(s) for: a. Blank b. Calibrator c. Sample X 6. Cuvettes. 7. Spectrophotometer. Methods : Procedure (A) : Estimation of Total bilirubin. 1. All the tubes was labeled accordingly to blank‚ calibrator and sample X. 2. 1500 µL of Total bilirubin was pipettes into each tubes. 3. All tubes was incubated at 37oC for 5
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concentrations 10µM‚ 7.5 µM‚ 5.0 µM and 2.5 µM then the absorbance of each sample was measured using a spectrophotometer. This generated curve with resulting average absorbances of 1.273nm‚ 1.0275nm‚ 0.585nm‚ 0.285nm and 0.124nm provided a means to determine the phenol red concentrations of two unknown samples after experimentally identifying the absorbance of the two samples using a spectrophotometer. 2. Based on the results in the standard curve exercise‚ the two unknown samples (unknown 1 and unknown
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and remained the same in about 35%. However‚ the worldwide piracy rate went up from 38% in 2007 to 41% in 2008. The global rate rose for the second year in a row because PC sales grew fastest in high-piracy rate countries such as China and India. (BSA‚ IDC‚ 2009) Among the lowest piracy countries‚ Taiwan has a higher piracy rate of 39% and loss of $201 million dollars. Ethics balances the social moral value and order with its invisible but powerful existence‚ and it is the guideline of behaviors
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Everybody grows up for a reason; a reason that parents would consider as blessings. Many parents would consider their children as their heirs and the ones who will work and take care of them when the time comes that they retire in their respective jobs as they grow old. They expect their children to be successful and have a good career condition. Career is a goal that came out from a long time desire and ambition which it is not affected by time or money. A career that yields great income does not
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equation‚ which will be related to how much NADH is present. We will then determine LDH activity by using the formula: ([ A/min]/6.22) x 78‚780‚ where [ A/min]=(initial absorbance-final absorbance)/3. We will be testing the absorbance using a spectrophotometer‚ which simply generates light that passes through a filter that causes the light to have a specific wavelength. NADH absorbs light maximally at a wavelength of 340 nm. Therefore‚ the measurement of NADH degradation is used to monitor the rate
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Effects of Facebook Addiction in the Study Habits of 2nd Year BSA Students of GenSantos Foundation College Incorporated In Partial Fulfillment of Requirements in Technical Writing Members: Averilla‚ Angelie Baisac‚ Julie Ann Estrebilla‚ Reah Giducos‚ Richie Subang‚ Kristia Lou March 2011 ACKNOWLEDGEMENT As researchers‚ we would like to extend our debt and gratitude to the following person who has been part of completing this requirement. We would like to direct
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Biology‚ Bio 110; October 1‚ 2014 Observing Membrane Structure and Observing Effects of Chemical stress on Membrane Crystal Eve Lopez‚ Dr. Barua Madhabi Keywords: beet root model system‚ spectrophotometer‚ betacyanin‚ cellular membrane‚ phospholipid Abstract The cellular membrane separates and protects the cell acting almost as a wall. Depending on what stressors there are the cellular membrane can become damaged. The objective of this experiment was to examine the struc
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purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally Affinity Chromatography on a Cibacron blue Sepharose stationary phase. Using BSA‚ which is analogous for BCA assays‚ a standardization was created to understand where the protein concentration was for each fraction. Introduction: This experiment is a continuation of the lab’s efforts to purify L-LDH. Previously our enzyme was purified
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the 7 mL chloroplast was prepared by pipetting the 7 mL amount of the chloroplast in to the clean‚ dry cuvette. A spectrophotometer was warmed up from the beginning of the lab to get prepared for the actual testing. A blank sample which contained 7mL sorbitol buffer was used to celibate the device between each different wavelength. the result shown on the spectrophotometer was recorded. Filters Filters In order to observe the activity of the chloroplast in different wavelength of the
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The Growth of Bacteria Influenced by Different Antibiotics Introduction: Plants are made up of three basic kinds of tissue that help it carry out basic functions that are necessary to maintain life and proper photosynthesis. The first and outermost layer of tissue is called the dermal layer. This is the plant’s ‘skin’ and serves the plant mainly as a source of protection. Also present in the dermal layer of the leaves are stomata‚ which are tiny openings that allow carbon dioxide to enter
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