Experiment 2: Starch Hydrolysis by Amylase Theoretical Background Polymers of carbohydrates are called polysaccharides‚ and make up some of the most important naturally occurring compounds [1]. They have thousands of monosaccharide units linked to each other by oxygen bridges. They include starch‚ glycogen‚ and cellulose‚ all three of which yield only glucose when completely hydrolyzed [2]. A B Figure 1. Starch (amylose) (A) and cellulose (B) Starch
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the protein or not1. However‚ the point of this lab was to show how light can be used to determine the concentrations of either DNA or proteins in solution‚ which is a very important part of virtual drug screening. This lab introduced how a spectrophotometer can be used to determine the absorbance of different concentrations of a certain solution‚ and then how to use the information gathered and Beer‟s Law to calculate the concentration of an unknown solution. Beer‟s Law relates the length which
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alpha-amylase and starch was predicted to be a temperature of 50°C and a pH of 5. The optimum temperature and pH for the reaction was determined by monitoring the reaction rate of alpha-amylase at different temperatures and pH’s by means of using a spectrophotometer to measure the disappearance (in absorbance) of the substrate starch. As a result‚ the absorbance of the substrate starch decreased at different rates for each temperature and pH as time continued to increase. The results showed that the reaction
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Kathleen Kramas Cells and Heredity Lab Tuesday 2:00-4:50 22 OCTOBER 2012 Membrane Permeability Decreases as Molecular Size Increases Introduction: Red blood cells are vital to organisms functioning properly. They are microscopic cells that carry oxygen from the lungs to all the tissues throughout the body. Upon transporting oxygen‚ red blood cells also exports waste‚ such as carbon dioxide‚ to the lungs where it can be expelled. Red blood cells are made up of hemoglobin which is surrounded by a
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B. Provider Bias and Inequality of Care Wang et al. (2001) conducted a study‚ which found that African Americans were relatively unlikely to receive guideline-based care. Gaps in quality encompass the elderly and seriously mentally ill African Americans. Many African Americans in the study were frustrated by the narrow focus on medications among mental health professionals. The medication-centered focus of the clinician clashed with the focus of the participant (Carpenter- Song et al‚ 2010). Finally
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absorbed by a substance dissolved in a non-absorbing solvent is directly proportional to the concentration of the substance and the path length of the light through the solution. In order to find the solutions absorbency‚ we must first use a spectrophotometer which is a machine for determining the ability of a solution to absorb light of a specific wavelength by measuring the transmitted light. This must be done first in order to find a solution’s absorbency. By using the standard solutions previously
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ESTIMATION OF IRON IN IRON ORE-SPECTROPHOTOMETRIC METHOD By: Taylor Villari Experiment conducted on 7/22/13 Components of each test tube examined in the spectrophotometer Trial | Volume of Iron solution (mL) | Micrograms of Iron | Volume of 10% sodium acetate | Volume of 0.1% o-phenanthroline | Volume of water (mL) | 1(blank) | 0.0 | 0.0 | 1.0 mL | 1.0 mL | 8.0 mL | 2 | 1.0 mL | 10 | 1.0 mL | 1.0 mL | 7.0 mL | 3 | 3.0 mL | 30 | 1.0 mL | 1.0 mL | 5.0 mL | 4 | 5.0 mL | 50 | 1.0 mL
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to determine the concentration of hemolymph in the body of a cockroach. It was done by making serial dilutions to make a standard graph by injecting a dye into a cockroach and determining the concentration at different times with the use of a spectrophotometer to find the optical density which is measured in absorbance. Results showed that the concentration was not within the pre-experiment research range. Introduction: Cockroaches as well as all other insects have an open circulatory system. Unlike
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DNA extraction and purification is an essential tool in understanding the biological basis and significance of a eukaryotic cell. Its role is pivotal to numerous scientific applications ranging from basic science research to applied research. Hence‚ DNA is found in all living organisms. Fragaria x ananassa‚ also known as a strawberry has been widely used as one of the many biological models in studying DNA structures due to its accessibility. Strawberries are octoploid which contain large genomes
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Chapter 3 RESEARCH METHODOLOGY Introduction In this chapter the research methodology used in the study is described. The geographical area where the study was conducted the study design and the population and sample are described. The instrument used to collect the data‚ including methods implemented to maintain validity and reliability of the instrument‚ are described. Research Approach and Design Denzin and Lincoln (2000) suggest a different‚ more complex definition. Their definition‚ which
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