_______________________ KIM MONICA V. POLLO President‚ BSA 1-133 Noted by: _______________________ MR. MARIO S. PELAGIO CWTS‚ Faculty Approved by: ____________________________ DR. ROVELINA B. JACOLBIA NSTP Director Republic of the Philippines Polytechnic University of the Philippines Sta. Mesa‚ Manila COLLEGE OF ACCOUNTANCY National Service Training Program Civic Welfare Training Service 2 PROJECT PROPOSAL I. Project Title: Tree Planting II. Proponents: PUP-NSTP CWTS 2-BSA 1-13 III. Duration: January 25‚ 2015
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Methods We began this experiment by turning the Spectrophotometer to 605 nm absorbance‚ and setting up each of the tubes as stated in Table 7-2*‚ ignoring the DPIP until the rest of the sample was set up. Once all of the samples were set up accordingly‚ we blanked the spectrophotometer‚ and added the DPIP to the sample right before‚ inverting the sample and taking a reading at the Zero time mark‚ and placing the sample in the designated light apparatus‚ Sample 1 was wrapped in aluminum foil to
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the process. Each enzyme has a region on its surface‚ called the active site‚ which recognizes a specific substrate molecule. The substrate is chemically attached to the active site and binds an enzyme-substrate complex. With the use of a spectrophotometer‚ the absorbance is recorded and the rate of reaction is observed when differing amounts of substrate and
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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Beer-Lambert law to explain the relationship linking concentration and absorbance of the coloured solutions methyl orange and bromophenol blue. • To be able to rightly operate a spectrophotometer to assess the absorbance at various wavelengths • To be able to identify the main component parts of a spectrophotometer • To be able to draw a ‘Standard Curve’ from the data collected • To be able to calculate the ‘percentage absorption coefficient’ for the solutions methyl orange and
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The College of Accountancy is one of Polytechnic University of the Philippines foremost college. It boasts of having produced topnotch in CPA Licensure Examination and accounting graduates who are holding prestigious and prominent positions in various companies‚ privates and government‚ all over the country and government‚ all over the country and even overseas. College of accountancy and Law has the highest enrollment among the colleges‚ institutes and schools of PUP. Besides‚ it is as old as the
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Materials ● ● ● ● ● ● ● Substrate solution Cuvette Spectrophotometer Timer Enzyme solutions Pipette for transferring fluids Thermometer Methods ● There is a choice of six different enzyme & substrate combinations in which the temperature will vary at which the reaction as well as the enzyme & substrate concentrations. pH: 1. A SUbstrate B/ Enzyme B was selected along with setting the wavelength to substrate vial/ 680 & the spectrophotometer to read light absorption. 2. The temperature was set to 25 degrees & set to different
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be allowed within the BSA program. I myself have no bad feelings about the LGBT community and even have a few LGBT friends in the program. The reason I believe what BSA is doing is good is due to the fact that within the program there has been a lot of fighting about letting the LGBT into the program. The program is now allowing diversity into the program which is also allowing those who have been at a difference be part of something new. Over the last few years the BSA program has been allowing
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continues to grow‚ according to the Business Software Alliance (BSA) and the Software and Information Industry Association (SIIA). In 1998‚ 38 percent of applications in use globally were pirated‚ down from 49 percent in 1994. Yet‚ 231 million business software applications installed were pirated‚ 2.5 million more than in 1997. This led to an eleven billion dollar loss in revenue by software companies (Paquet). Jason Penchoff‚ a BSA spokesperson‚ states‚ "Software piracy affects company productivity
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test tube pH 4B solution to blank the spectrophotometer. Then‚ a cuvette filled with the pH 4 test tube solution was inserted into the spectrophotometer and measured with the 420 nm wavelength. This procedure was repeated for the remaining test tubes. Exercise B required 4 test tubes labeled with “A‚B‚C‚ or D” and they held the solution of different pH buffer and potato juice volumes‚ and 1 mL of water (Table 4). These tubes were the blanks for the spectrophotometer for recalibrating it. The additional
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