Colorimetry Introduction Absorption Spectroscopic methods of analysis rank among the most widespread and powerful tools for quantitative analysis. The use of a spectrophotometer to determine the extent of absorption of various wavelengths of visible light by a given solution is commonly known as colorimetry. This method is used to determine concentrations of various chemicals which can give colours either directly or after addition of some other chemicals. As stated by the Beer-Lambert Law
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ANALYSIS OF ASPIRIN: SPECTROPHOTOMETRY The purpose of this experiment is to evaluate the percent aspirin on a commercial aspirin tablet using an instrumental method‚ spectrophotometry. In a spectrophotometer‚ light from a strong lamp passes through a monochromator‚ which breaks the light into its component colors using a grating‚ then uses mirrors and slits to direct a light beam of the desired wavelength through the sample compartment‚ where you place a tube (cuvette) of solution. A detector
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To construct a calibration curve‚ three samples with different known concentrations and a blank solution are prepared and their absorbances are measured with a UV-Vis spectrophotometer (Table 1). The absorbances of three unknown samples with same concentration and a blank are measured (Table 2). The blank solutions are used as a reference solution to calibrate the colorimeter. The volumes of Fe solution for the delivery volume errors in the 10 ml graduated pipet are corrected (Table 3). The molarity
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Effects of Temperature‚ pH‚ Boiling‚ and Hydroxylamine on the Enzyme Peroxidase Extracted From Brassica rapa Abstract In this experiment the enzyme peroxidase was extracted from from a turnip‚ Brassica rapa‚ and tested under different conditions. The effects of temperature‚ boiling‚ pH‚ and a competitive inhibitor were tested. The enzyme was tested at temperatures of 4°C‚ 24°C‚ 32°C‚ and 48°C. As the temperature increased‚ so did the activity of the enzyme
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Materials: 1Wax Marking Pens 150 ml Beakers 3 400 ml Beaker 1 container of parafilm 1 set of 20 spec tubes 1 regular test tube rack 1 small test tube rack 1 box Kimwipes Eye Droppers 1 thermometer 2-10ml Graduated Cylinders 1 Spectrophotometer 37 °C waterbath with test tube racks Solutions: 1 flasks of pH 7 buffered ONPG 1 flask of Lactose 8% 1
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Instant download! Use PayPal to get your Tutorial. PayPal will immediately take you to the download page‚ plus you will get a backup link INSTANTLY sent to your email! 100% SATISFACTION GUARANTEED! UOP-TUTORIALS.INFO BSA/375 Fundamentals of Business Systems Development BSA/375 Week 1 - Software Development Resources: University of Phoenix Material: Recording a Narration Read the following scenario: Senior management at your company is concerned about why so many software development efforts
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water. The four sources that could be the point of contamination are a new subdivision‚ textile plant‚ an organic farm‚ and a mountain lake. We had to find the concentration of each known standard and unknown standard. We did this by using a spectrophotometer. The results were the following‚ the organic farm with a herd of 50 cows and a 10 acre field of zucchini had the highest levels
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The Visible Spectra Of Orange Soda Pop Objective : To measure an absorbance value of the different colour solutions by using Spectrophotometry Introduction Spectrophotometry involves the use of a spectrophotometer. A spectrophotometer is a photometer (a device for measuring light intensity) that can measure intensity as a function of the color‚ or more specifically‚ the wavelength of light (nm). The instrument also can be use on biological sample such as chlorophyll pigments and
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Red Dye in Sodas Abstract The use of red dye #40 is common in various soft drinks today. The labels on these beverages do not specify how much dye we are consuming. We did this experiment to find out which soda uses the most dye. Using a spectrophotometer‚ we measured how much light is absorbed by various known concentrations of red dye. After collecting this data‚ a standard curve was made that correlated the concentration of red dye #40 to its absorbance rate. Our results showed that the sample
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Invertase was then mixed in different buffer solutions of varying pH. After subjecting the enzyme to the different buffers‚ absorbance of the invertase was measured under 540 nm using a spectrophotometer. Invertase was subjected to different PH of buffer solution and was observed under 540 nmabsorbance using spectrophotometer. After observation and analysis‚ a peak was observed by plotting absorbanceversus ph and was known as optimum PH. Optimum PH is said to be the most favorable PH value or the point
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