Determining the Absorbance Maximum Wavelength and Molar Extinction Coefficient to Find the Molarity of the Unknown DCPIP Background Information: A spectrophotometer is an instrument used to help determine the absorption spectrum of chemicals. It does this by reading the absorbance of the chemical at different wavelengths. All chemicals absorb light in their own distinct way. This distinction helps to identify unknown chemicals. The absorption of light within a chemical is also very important because
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Catecholase (an enzyme) to catechol (substrate) will result in the creation of Benzoquinone. Materials & Methods Adjusted the spectrophotometer with blank solution‚ then measured 1ml of Catecholase (potato extract) in the test tube B‚ using 1 ml graduate pipette and added 5 ml of distilled water. Protected the test tube with Parafilm and mixed the content. On the spectrophotometer‚ arranged the wavelength to 540 nm‚ formerly inserted the test tube into sample
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Proteins Biuret Test Results. Figure 1 is showing both graphs of results from the spectrophotometer. Figure 1A is the results without the unknown plotted. By using the best fit line and equation the unknown substance was found to have .75 absorbance and 11.5 mg/mL protein concentration. Figure 1B shows the newly found data of the unknown plotted
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May 2013 Election: Manual System or Automated Election System Introduction Elections have played an integral role in the development of one’s country. Essentially‚ an election is a procedure by which members of communities and/or organizations choose persons to hold an office. It is a technique of rendering authority and/or creating representative bodies. Elections are often linked to the idea of democratic representation. Therefore‚ an election is a device for filling an office or posts through choices
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BSC 2010C.LAB TH 7-9:50pm 29 August 2013 Biology Lab Report Lab #1 –PROTEIN EXTRACTION LAB I. INTRODUCTION To begin the process of protein extraction and compare the results in a study‚ it is necessary to understand the importance of proteins‚ the process of extraction and how you are using the results to determine a rational conclusion. First understand proteins and the necessity of studying their impact. Proteins are essential molecules for biological functions and are the
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simultaneously mixed. The reactions were then introduced to I2-KI‚ which stopped the reactions‚ at two minute intervals. Each of these trials was repeated three times to ensure proper accuracy. After concluding the reactions they were placed into a spectrophotometer (A580) for analysis. Graphing the values of the absorbance to time for each pH it was found that the rate of reactions in the neutral pH solutions were much higher than that of the acidic solution. We concluded that this was because of the possibility
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Chapter 1 Introduction Background of the Study Having been a former high school graduating students‚ we experienced the same stress and pressure of choosing amongst the multitude of course to take in college. In view of this things‚ we decided to conduct a research regarding what courses will further their chance of getting a good career upon their graduation. We will make a parallel research having the pulp of the presently graduating 4th year students regarding their course
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team‚ but each person has their own unknown and part of the score depends on you accuracy. Procedure: The spectrum of a carbonated beverage is obtained by measuring the absorbance of a sample of the beverage at different wavelengths using a spectrophotometer. This spectrum can then be related to the color of the beverage. In the second part‚ a calibration curve is prepared by measuring the absorbance of different standard concentrations of grape soda at a single wavelength. The absorbance of the
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determine their own antidiscrimination policies. And that is how Larry Norvell (local head of the UW of Columbia) had to face the issue of allocations to the local BSA organization -- Cascade pacific Council of the Boy Scouts of America (CPCBSA). The central dilemma for Norvell is whether or not to address the discriminatory actions of the BSA and CPCBSA organizations and what the impact of this decision will mean to his organization stakeholders (e.g. board members‚ UWCW personnel) as well to other
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Title Biochemical Analysis of Blood Abstract Introduction After blood is collected from a patient it is analysed to check the fragments present and the fluid which will either be plasma or serum. After plasma is separated from blood it recollects the fibrinogen which in turn helps in clotting however serum is the portion of blood which remains after the fibrinogen is removed. Serum contains approximately 6-8% of the proteins which make up blood however plasma creates up to 55% of blood
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