Abstract This lab was performed in order to discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close
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Assays 1. (4 points) Compare and comment on the concentration values calculated using the spot method vs the OD method with the spectrophotometer. Which do you think is more accurate? Why? Although I can not compare the concentration values between the spot method and the OD method‚ due to the pipetting error in the spot assay‚ the OD method with the spectrophotometer was more accurate due to the smaller possibilities of error. To add on‚ the spectrometer is a quantitative research‚ which means
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Connie Jamieson | 3/1/2012 | | Abstract Lysozyme is an enzyme found in both tears and saliva. Each time we blink our eyes‚ our eyelids wash tears over the surface of our eyes. In addition‚ our mouth is continually producing saliva. A spectrophotometer set to 450nm was used to measure the effect of lysozyme on bacteria. The absorbance or optical density was measured for each sample at specific times with a total measuring time of twenty minutes. In this experiment‚ it was shown that lysozyme
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with 3‚5 dinitrosalicylic acid(DNS) which produce a red-brown product Miller(1959)The reaction is direct‚thus the method is preferred over the Benedict’s test method. The concentration of the coloured complex can be determined with the spectrophotometer at Absorbance 540.The sugar concentration of unknown sample can then be read off a calibration curve(standard curve) created using known sugar concentrations.The dilutions of a solution of known concentration are used to determine the concentration
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grown in various carbohydrate solutions at various temperatures. In the second experiment‚ succinate was added to various samples of a mitchondrial suspension‚ DPIP‚ and a buffer. Then after two blanks were used‚ the samples were placed into the spectrophotometer for transmittance testing. Introduction Cellular respiration is a group of reactions that occur when a cell turns the energy from food and nutrient sources into ATP‚ releasing the rest of the products as waste. It is a catabolic set of reactions
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Abstract : The purpose of the experiment was to determine the wavelength and light intensity effect on the reaction rate of photosynthesis. Photosynthesis is the conversion of light energy to chemical energy in the form of organic compounds. There are two phases in photosynthesis‚ the light reaction and dark reaction. The following experiment tested only the light reaction. Photosynthesis takes place on the chloroplast‚ and the chloroplast contains chlorophyll pigments which absorb light energy
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Local Studies Students Performance under retention policy In 1998‚ University of the East College of Business Administration under her leadership was listed by the Professional Regulation Commission (PRC) as the number 3 top performing school with over 100 examinees. This continuous performance is the reason that it granted a second level re-accreditation by The Philippine Association of Colleges and Universities Commission on Accreditation (PACUCOA). Together with the retention
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largest in the university. It has a well selected teaching staff able to provide students with excellent business education. One of the curriculum programs that it offers is the Bachelor of Science in Accountancy. Bachelor of Science in Accountancy (BSA) prepares students for entry into a variety of fields in accountancy‚ including corporate accounting‚ accounting information systems‚ and governmental accounting‚ as well as entry into graduate programs such as law. It also equips students with basic
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Micah Bolivar MWF 8:30-9:30 ULTRAVIOLET-VISIBLE SPECTROMETRY A diagram of the components of a typical spectrometer are shown in the following diagram. The functioning of this instrument is relatively straightforward. A beam of light from a visible and/or UV light source (colored red) is separated into its component wavelengths by a prism or diffraction grating. Each monochromatic (single wavelength) beam in
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prepared after acid wash of the glasswares Glassware * 2 Beakers * 11 conical flasks * 9 Volumetric flasks 50 ml * 1 Volumetric flasks 1000 ml * Pipette type 1 * Pipette 25 ml * Stir rod * 11cuvette * Spectrophotometer Chemical needs; hazards and safety precautions: 1. Sulphuric acid It is very hazardous in case of skin contact. It is classified as corrosive‚ irritant and permeator. Safety needs: Lab coats‚safety goggles and gloves should be worn to
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