fifteen percent‚ and five percent concentrations of NaCl. Three different treatments of ortho-nitro-phenyl-galactoside‚ lactase‚ and perspective salt concentrations were prepared in cuvettes where the reaction would be observed and recorded from a spectrophotometer. Larger concentrations of salt adversely effect the reaction activity
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Angelica C. Mendoza BSA 1-1 Reaction Paper Educational tour turns a day of recreation. September 23‚ 2011‚ PUPians need to wake up early. More or less 2:00 in the morning just to come to our campus exactly on time. It is written to our waiver that 4:00 is our pick up time. Gathering my bag filled with my snacks‚ drinks and extra cloths‚ I went to my beloved school with an excited feeling. Time runs very fast. We’ve waited for our bus‚ Bus 1
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hypothesis can be made that insulin given to a subject with type 1 diabetes would help lower his or her blood glucose levels while insulin that is given to a normal subject will have no effect. Materials and Methods Experiment 1: • Spectrophotometer • Centrifuge • Incubator • 5 test tubes • Pipettes • Tweezers • 1 bottle of Glucose Standard • 1 bottle of deionized water • 1 bottle of Enzyme Reactor Agent 5 test tubes were filled with glucose
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ubility * 1 To measure the molar solubility of a sparingly soluble salt in water. * 2 To prepare a calibration curve based on complex ion formation for absorbance enhancement. * 3 To calculate the solubility product constant (Ksp) of a sparingly soluble salt from its molar solubility. * 4 To confirm the common ion effect on the molar solubility of a sparingly soluble salt. Introduction In previous introductory chemistry courses‚ you learned some basic solubility rules that are
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It was also accounted for human error that might happen during the experiment. For the BSA standard‚ the concentration increased as it went down from row A to G in the column 11 and 12. It made sense that the stage 1 enzyme would have blue color due to the amount of other protein that had not been separated. The well color of stage 4 enzymes
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I. Objective: The objective of this experiment is to determine the mass percent of iron in an iron compound using a spectrophotometer. From there‚ determine which iron compounds are in the stock room bottles based off of the experimental mass percent results. II. Introduction: The objective is to determine the mass percent of iron in an iron compound using a spectrophotometer. From there‚ determine which iron compounds are in the stock room bottles based off of the experimental mass percent results
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Miskiewicz 2006). A high photosynthetic rate can be interpreted by a fast rate of change from blue to colourless of the DCPIP as more electrons are flowing through the transport chain and reducing the DCPIP. The colour of DCPIP is measured using a spectrophotometer at 605nm. The rate of photosynthesis is dependent on many factors‚ in particular light quantity (Johkan et al. 2012). A greater quantity of light received by the chloroplasts equates to a greater amount of solar energy potentially converted
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test tubes (ethyl acetate soluble fraction‚ ethyl-3-hydroxy-5-methoxy-4-methylbenzoate‚ ascorbic acid‚) labeled separately were shaken well and incubated in the dark at 25oC for 30 minutes. Then the Kinetic absorbance was recorded at 734 nm by spectrophotometer after 1 and 6 minutes for each concentration and mean was taken for each reading. The potential to scavenge the ABTS radical was calculated using the following
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Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how it is done‚ 6CO2 + 6H2O + light
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Peptide synthesis – chemistry and modifications Peptides and proteins exhibit the largest structural and functional variation of all classes of biologically active macromolecules. Biological functions as diverse as sexual maturation and reproduction‚ enzyme inhibition‚ blood pressure regulation‚ glucose metabolism‚ thermal control‚ analgesia and learning and memory are now thought to be regulated by peptides. Peptide synthesis chemistry Synthetic peptides are valuable tools in analysis of
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