the end of the tips contain quartz to calculate the absorption maximum. 3. The result of absorption maximum from the graph was printed. 4. Absorption maximum will be used to determine the unknown concentration. D. Operation of the UV-VIS spectrophotometer 1. Cary win UV was selected and
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New Era University Research Paper Submitted By: Joseph Jacinto V. Abaoag 1- BSCOA 5 ENGLISH 2 Fri 1:00-4:00 Chapter I “I like cars a lot. I and my friends started stealing cars over a year ago. I didn’t want to do it but we all felt we could have some fun and get away with it... I’ve been caught twice by the cops and they say if I do this again I will go to jail. I don’t like what this has done to my mom and dad. They don’t want me to hang with these guys anymore but they’re my
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test tubes‚ including 1 blank‚ with different amounts of pH 5 buffer‚ H2O2‚ Peroxidase‚ and Guaiacol. After the certain amount of mL per substance was mixed‚ the absorbance readings for the effect of peroxidase concentration were taken from the spectrophotometer. The results for the effect
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Using the Phenol-Sulfuric Acid Method This lab introduced the students to analyzing carbohydrate content using a spectrophotometer and phenol-sulfuric acid methods. Virginia Smith 983976633 Lab Partner: Jeff Martz Due Date: 3-22-11 Introduction The objective of this lab was to have students use a spectrophotometer to analyze the carbohydrate content in the sample. Phenol-sulfuric acid methods were also applied to determine the total
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Plant Pigments and Photosynthesis Problem: We are going to separate and identify pigments and other molecules from cell extracts through a process called chromatography. We will also test the theory that light and chloroplasts are required for light reactions to occur. Background: Pigments are chemical compounds which reflect only certain wavelengths of visible light. This is what makes them seem colorful. Many things contain pigments including flowers‚ corals‚ and even skin. More important than
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determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume ( 50 µl) of
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Human Resources in Riordan Manufacturing Jeremiah Macon Jr.‚ Benita Christian‚ Marietta Esquibel‚ Trey Robledo‚ Eric Sedgley BSA/502 December-24-2012 Lise Hautzinger Human Resources in Riordan Manufacturing When people think of the responsibilities of a human resources department‚ they may say they are responsible for the hiring and firing of employees. The truth of the matter is that a human resources management is responsible for much more than just hiring and firing. The human resources
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governments developed arrangement and legal codes to deal with software piracy. Governments have been passing and enforcing laws supporting copyrights. The effort to condemn software piracy led to more governments joining the effort‚ associations such as BSA (Business Software Alliance) have members in more than 70 nations. All this effort can’t really provide significant results in the future. Other method has to be considered as well. Narrowing the gap in price of the legal and illegal copies‚ my encourage
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Four environmental factors of enzymes were tested in lab. The changing of pH‚ substrate concentrations‚ temperature‚ and an inhibitor (NaCl) and the effects it hade on the enzyme turnip peroxidase. Enzymes are biological catalysts which increase reaction rates by lowering the activation energies of substrates. A substrate is a reactant that interacts with the enzyme. The enzyme and substrate can be viewed as the recently discovered "induced fit model"‚ which suggests enzymes are flexible and dynamic
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the absorbances corresponding to the following values of the percentage of transmitted light: (Provide your final answer with three decimal places) a. 95% b. 88% c. 71% d. 50% e. 17.5% f. 1% 2. A solution of a compound (1.0mM) was placed in a spectrophotometer cuvette of light path 1.05cm. The light transmission was 18.4% at 470nm. Determine the molar extinction coefficient. Include units in your answer. 3. The molar extinction coefficient of reduced NADH (nicotinamide adenine dinucleotide phosphophate)
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