Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results 1 2
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awards here at Xavier‚ for exemplifying excellence in leadership‚ involvement‚ service‚ and a history of significant contributions to the Black Student Association and the Xavier University community. Recently in January 2017 when a couple members of BSA‚ ASA‚ NPHC went to the African American Leadership Experience held in Washington‚ DC‚ I witnessed as she was given the Dr. Carroll F.S. Hardy Legacy award out of students from approximately fifty different American schools that were present. This
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References: Bernstein‚ C. (1992). The idiot culture. The New Republic‚ 8‚ 22-28. Berry‚ D. (2009). Journalism‚ ethics and society. UK: Ashgate. Bok‚ S. (1982). Secrets: On the ethics of concealment and revelation. NY: Pantheon. BSA. (2000). Decision Nos 200-108-1113 (10.8.00). In the matter of complaints by W.DeHart‚ L.Cameron and P. & P. Cotter. A full copy of this Decision is available at www.bsa.govt.nz. Burns‚ L.S. (2002). Understanding journalism. London: Routledge. Cheng
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1. Describe the mechanism by which green fluorescent protein (GFP) is able to emit fluorescent light and why ultraviolet light is required to visualise it. (5 marks) When GFP is hit with UV light‚ the chromophore is hit by a photon. This changes the chromophore from ground state (A) to A*‚ which is a highly excitable state. Due to such a highly excitable state not being able to remain so for very long‚ the A* state chromophore emits a proton‚ lowering its state to I*‚ the energetic I. This I* state
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1279- 1212 BC)- at El-Assasif district‚ El-Qurna necropolis (Luxor-Upper Egypt). The visible reflectance spectra of the samples were obtained by a Miniscan® XE Plus spectrophotometer (HunterLab). The reflectance spectra were registered in the visible wavelengths domain (from 400 to 700 nm). Chromatic values are expressed as color coordinates in the CIE L*a*b* color system (1976) under the standard illuminant D65 and a 10º/observer
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were placed in the spectrophotometer to determine the percent transmittance of FD&C Blue 1. All of the data was summarized in graphs to predict the concentration of FD&C Blue I in a sample of Gatorade. Materials: FD&C Blue 1 stock solution‚ 50-mL Sample of consumer sports drink‚ 10-mL Water‚ distilled or deionized Beakers‚ 50-mL‚ 2-3 Cuvets or test tubes‚ 13 x 100 mm‚ 3-8 Kimwipes or lens tissues Pipet‚ serological‚ 10-mL Pipet bulb or pipet filler Spectrophotometer or colorimeter
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Kit‚ three different samples of 100 uL of isolated A. fischeri chDNA solution each were obtained. Next‚ to determine the purity and concentration of the isolated A. fischeri chDNA samples‚ UV spectrophotometry was performed using two different spectrophotometers. The best sample was chosen for the next step in the experiment‚ restriction digestion‚ which had a concentration of 196.6 ug/uL. Purity analysis shows a 1.83 for the ratio of A260/A280 and a 2.15 for the ratio of A260/A230. Both of these values
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The Spectrophotometric Determination of an Equilibrium Constant. Abstract: The report presents determination of equilibrium constant for the formation of a complex ion FeSCN2+. This was accomplished using a colorimeter to measure absorbance of some known concentration solutions in order to generate the calibration curve. The equation of the graph was used to compute the equilibrium concentrations of the reactants and products‚ needed to calculate the equilibrium constant for
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pH Affecting on Amylase Enzyme Is there a difference in the concentration of pH in the amylase enzyme activity? The null hypothesis would be that there will be no difference in the concentration of pH and alternative hypothesis would be that there will be a difference in the concentration of pH. The predication if that there will be a difference in the concentration of pH. The pH affects on enzyme activities‚ one of the enzyme that was used in the process was amylase. When a serial dilution was
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JUDY ANN D. ALEJANDRE BSA-III CHAPTER 2- Activity 1 1. Bench is known for their fashionable and yet affordable wearing apparels not just for men but also for women. Ben Chan introduced their products not just in the Philippines but also in other countries. They focus on their customer’s needs and wants. They are able to satisfy the different types of customers. 2. Many consumers nowadays are into fashionable yet affordable clothing/apparel. Because of this‚ Bench introduced their fashionable
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