Results The objectives of this experiment were to investigate diffusion of molecules across a membrane and the factors that affect the rate of diffusion. The hemolysis time of sheep erythrocytes was measured for each of eight different nonelectrolyte solutions by eleven groups in BI 108 Section D2. These eight nonelectrolytes were urea‚ thiourea‚ methanol‚ ethanol‚ propanol‚ ethylene glycol‚ diethylene glycol‚ and triethylene glycol‚ and the mean hemolysis times can be seen below in Figure 1. The
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Hui Tzu(Erin) Wang ID:0720052 Effect of an Increasing Substrate Concentration on Enzyme Activity Rate Abstract The reaction rate of an enzyme can be affected by many factors‚ and the purpose of this experiment was to find out how an increasing substrate concentration influences the rate of an enzyme activity; we obtained data from recording the absorbance of the samples which contain the same amount of potato juice (enzyme oxidase) and different
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Are there any differences in the rate of metabolism of a dried yeast culture with differing carbohydrate sources? In the current practical that was undertaken the growth rate of yeast (S. cerevisiae) with differing carbohydrates sources : Glucose (C6H12O6)‚ Fructose (C6H12O6)‚ Lactose (C12H22O11)‚ Xylitol “(CHOH)3(CH2OH)2” and Water (H2O) as a Control were observed. “ Yeast are single-celled fungi which consist of more than one thousand different species which have been identified. The most commonly
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primarily responsible for photosynthesis. They capture light energy to conserve free energy in the form of ATP and reduce NADP to NADPH. (tb) The rate at which photosynthesis happens can be measured using a dye (DCPIP)‚a Hill reaction‚ and a spectrophotometer. ( DCPIP ) 2‚6-dichlorophenolindophenol is a compound that is often used as a redox dye. Oxidized DCPIP is blue‚ DCPIPH‚ which is reduced by a single electron‚ is pink‚ and fully reduced DCPIP is colorless. It is often used in measurements of
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solution of a known concentration was diluted to create a dilution series‚ then placed in a spectrophotometer set to 525 nm. The absorbance of the dilution was used to create a standard curve for betacyanin. Discs of living beet roots were then placed in water and exposed to various temperatures. A sample of the water that the beets were in was taken and absorbance was determined by the spectrophotometer and concentration from the standard curve for betacyanin. Findings: In comparison to the control
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Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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Journal of Medicinal Plants Research Vol. 5(10)‚ pp. 2035-2040‚ 18 May‚ 2011 Available online at http://www.academicjournals.org/JMPR ISSN 1996-0875 ©2011 Academic Journals Full Length Research Paper Screening of chemical composition of essential oil‚ mineral elements and antioxidant activity in Pulicaria Undulata (L.) C. A. Mey from Iran Mehdi Ravandeh1‚ Jafar Valizadeh2*‚ Meissam Noroozifar1 and Mozhgan Khorasani-Motlagh1 1 Department of Chemistry‚ University of Sistan and Baluchestan
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area. The objective of this study was to observe how temperature disrupts the membrane function in living beet cells. One aspect of this included a comparison of the amount of betacyanin that is released in different temperature treatments. A spectrophotometer will be used to measure this variable by measuring the absorbance value of the betacyanin in solution after each treatment. The results of this study will show the necessary temperature to keep the vacuole intact. It would influence common agriculture
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Overdosing and under dosing‚ both are of worry with 5-FU‚ despite the fact that studies demonstrate that the lion’s share of colorectal growth patients treated with 5-FU are under dosed in light of today’s dosing standard‚ body surface range (BSA). The confinements of BSA-based dosing keep the oncologists from precisely titer the measurement of 5-FU for the dominant part of individual patients‚ which brings about unnecessary danger or treatment
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copper. The following report will walk you through my actual findings. Our lab consisted of three parts: determining the proper wavelength for the spectrophotometer‚ constructing a calibration curve for Cu(NH3)42+‚ and finally determining the actual content of copper in a penny. IN the first step of the lab‚ we used water to calibrate our spectrophotometer‚ and then measured the absorbance of Cu(NH3)42+ at different wavelengths‚ in order to determine the optimal one. In our group‚ we determined 600nm
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