phosphate buffer pH6 containing the enzyme: polyphenol-oxidase‚ was used as the variable being tested in this experiment. You may refer to the Enzymatic Reactions Biology 21 Lab Manual. Santa Monica College by Logan‚ R. 2003 to see how the potatoes extract in phosphate buffer was made. We began this experiment by measuring seven constant amounts of 1ml of 0.1% catechol using a 1 mL pipet into each seven cuvettes. The catechol is our substrate solution. Next‚ different amounts of phosphate buffer ph.6
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Sodium phosphate Dibasic‚ which equal to 3.549g; we added 50.0ml of water to be dissolved. We made these two solutions in order to get their PH. We started with PH 6.0 buffer from Sodium phosphate monobasic solution‚ we added 50ml of Sodium phosphate Dibasic to 250ml beaker‚ placed PH probe‚ then added solution Sodium phosphate monobasic until PH was between (5.9-6.1)‚ and we called it solution
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of each pH buffer to insert into cuvettes‚ a micropipette was then used to obtain 0.5ml of catechol and 0.5ml of the catechol oxidase. The pH buffer was made first to avoid any denaturation of the catechol oxidase. Our positive control for this experiment was pH 7 because that is the pH level of most cell membranes in the cytoplasm (Whitson‚ 2016.) Our negative controls varied for each pH buffer‚ but all “blank” cuvettes contained 0.5 ml of catechol oxidase and 4.5ml of the pH buffer. We prepared
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Law‚ which is the linear relationship between absorbance and concentration of an absorbing species. Absorbance formula is shown in fig. 1.1. However‚ the Beer-Lambert Law is not obeyed at high concentration as solution with high concentration may alter the refractive index of the solution which in turn may affect the absorbance reading. The limitation of the Beer-Lambert Law is shown by the non-linear part of the graph in fig 1.2. The absorbance
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resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of water. Five more solutions were prepared in the same fashion except without the 1.0mL of water. These five experimental solutions were capped
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of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate. The three solutions used contained the same amount of mitochondrial
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To begin the experiment‚ 8 ml of 0.1% Bromophenol blue (BPB) solution was made by diluting 0.25% BPB solution. From the 0.1% BPB solution‚ six diluted solutions‚ ranging from 1:50 to 1:10000‚ were prepared. Each solution was then run in the UV spectrophotometer to measure the absorbance at 590nm. DI water was used as a blank and samples were measured starting from the least concentrated one. The graph of concentration versus absorbance was plotted from the obtained data. In the second part‚ each
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1. The standard curve is a graph that shows a relationship between concentration and absorbance of a solution. A standard curve was experimentally created in this experiment using 10mL solutions of phenol red with concentrations 10µM‚ 7.5 µM‚ 5.0 µM and 2.5 µM then the absorbance of each sample was measured using a spectrophotometer. This generated curve with resulting average absorbances of 1.273nm‚ 1.0275nm‚ 0.585nm‚ 0.285nm and 0.124nm provided a means to determine the phenol red concentrations
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conduct my experiment‚ I measured 10g of ascorbic acid powder and mix it within 1 litre of water. I believed that by having the solution prepared before putting in the d-block elements would save time overall. Unfortunately‚ the data was all over the place‚ this is because the later I conduct the experiment‚ there is a higher chance of oxidation occur in the ascorbic acid solution as it have a direct contact with oxygen in the atmosphere. In order to keep constancy‚ I had to reduce the amount of ascorbic
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most suitable for this analysis is the Solution-Focused Theory. Solution- Focused Theory is a combination of several approaches‚ that focuses heavily on the present and future. When using this therapy is it not ideal to focus on past problems because it redirects the client thoughts‚ making them focus more on the problem then the solution. This therapy allows clients to set clear goals at the beginning of the counseling sessions (Lecture 7‚ 2012). The solution focused therapy includes identify questions
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