England Buffalo High School Abstract In this lab‚ the Beer-Lambert Law is tested by using a spectrophotometer. This is a quick and easier way to determine the concentration of a solution. Keywords: Beer-Lambert Law‚ Spectrophotometer‚ concentration Beer-Lambert Law The Beer-Lambert-Law states that there is a direct correlation between the concentration
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have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH nullifies the negative charge on the protein molecules. The pH of Buffer II is 4.7‚ which is <
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[OH-] in 0.025 M HCl ? 3. What is the pH of 0.50 M Sr(OH)2 ? 4. The pH of an aqueous solution is 10.32. The [OH-] is …………………… 5. The [H+] in 0.050 M Mg(OH)2 at 25oC is ………………………. 6. The pH of 0.20 M HNO3 is ……………………. 7. A solution is prepared by adding 200. mL of 0.125 M of HCl to a 500.0mL volumetric flask and filling it to the mark with water. The pH of this solution is ………. 8. Consider the equilibrium for an indicator: Hind(aq) + H2O(l) ⇌ H3O+
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vinegar solution has a H3O+ concentration of 0.015 M. What is the pH of the solution? a) 1.8 b) -1.8 c) 0.015 M d) 3.3 e) -3.3 2. What is the pOH of the solution in Problem #1? a) 10.7 b) 17.3 c) 12.2 d) 15.8 e) 7.0 3. The Ka of acetic acid is 1.8 x 10-5. What is its pKa? a) 1.8 x 10-5 b) 5.18 c) 4.74 d) -4.74 4. What is the pH of a 0.100 M solution of acetic acid? a) 1.34 x 10-3 b) 2.87 c) 1.00 d) 4.74 e) 3.74 5. What is the acetate ion (Ac- or CH3COO-) concentration of the solution in Problem
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P2O5 (B) PO43- (C) H2PO4- (D) H3O+ (E) H3PO4 Acid/Base Strengths 9. Given that HX is a stronger Brønsted acid than HY in aqueous solution‚ which is true of a 1 M solution of NaX? (A) It is less basic than a 1 M solution of NaY. (B) It is more basic than a 1 M solution of NaY. (C) It yields a neutral solution. (D) It is more concentrated than a l M solution of NaY. 10. Which
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Guidelines for writing laboratory reports Laboratory reports should be written according to the format below (failure to do so will result in marks being deducted): Formatting Font Type: Times Roman Font size: 12 Spacing: 1.5‚ justified Pages : 5 (minimum) - 10 (maximum) [pages must be numbered] Title page You are required to use the lab report submission page available on the LMS and are to include these details: lab no.‚ title of experiment‚ students’ names and ID‚ date of experiment as well
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and measure the enzyme activity using spectrophotometer respectively. For Experiment 5‚ a mixed solution of ONPG solution and Sodium phosphate buffer pH 7.3 is incubate at different temperature‚ 25‚ 40‚ 50‚ 60 and 70 °C. The mixed solution is then incubated for another 10 minutes after the enzyme is added in their respective temperature. Then‚ using spectrophotometer‚ the absorbance in the mixed solution incubated in different temperature is recorded. For Experiment 6‚ 5 different
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The dissolution of the drug in different liquisolid formulations was performed in 500 ml simulated salivary fluid (phosphate buffer pH 6.8) maintained at 37 ± 0.5 ºC using the USP dissolution tester apparatus II‚ at a rotation speed of 50 rpm. Aliquots from the dissolution medium were withdrawn at 1‚ 2‚ 3‚ 4‚ 5‚ 6‚ 7‚ 8‚ 9‚ 10‚ 12.5‚ 15‚ 20‚ 25 and 30 minutes time intervals. The samples were changed with fresh dissolution medium of same amount to maintain a constant volume in the vessels. Samples
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Biochem. J. (1995) 305‚ 17-20 (Printed in Great Britain) 17 RESEARCH COMMUNICATION The effect of low temperatures Nicole MORE‚ Roy M. DANIEL* and Helen H. PETACH on enzyme activity Thermophile Research Unit‚ University of Waikato‚ Private Bag 3105‚ Hamilton 2001‚ New Zealand The stability of two enzymes from extreme thermophiles (glutamate dehydrogenase from Thermococcales strain ANI and f‚- enzymes‚ glucosidase from Caldocellum saccharolyticum expressed in Escherichia
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ml Beaker 1 container of parafilm 1 set of 20 spec tubes 1 regular test tube rack 1 small test tube rack 1 box Kimwipes Eye Droppers 1 thermometer 2-10ml Graduated Cylinders 1 Spectrophotometer 37 °C waterbath with test tube racks Solutions: 1 flasks of pH 7 buffered ONPG 1 flask of Lactose 8% 1
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