proven that pH buffer 7 is the optimum pH for catalase in potatoes. This is because the height of the bubbles was the highest of all three tests (pH 3‚ pH 7‚ pH 9) reaching an average height of 0.433 cm. See graph 3. While pH buffer 3’s average height was 0.233 cm only reaching a maximum height of 0.3 cm in Trial 3 and pH buffer 9’s average height being 0.333 cm only reaching a maximum height of 0.4 cm again in Trial 3. See table 1. Therefore this supports my hypothesis: At pH buffer 7 the enzyme
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Once the buffer had mixed with the substrate we removed 500 microliters of this new solution and added it to the "Start" cuvette. Using a clean tip‚ we pipetted 1 mL of enzyme into the 15 mL "Enzyme Reaction" conical tube‚ gently mixed‚ and then started our timer
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General College Chemistry LabPaq / Published by: Hands-On Labs‚ Inc. sales@HOLscience.com / www.HOLscience.com / Toll Free 866.206.0773 A Laboratory Manual of Small-Scale Experiments for the Independent Study of General College Chemistry 50-0123-CK-02 LabPaq® is a registered trademark of Hands-On Labs‚ Inc. (HOL). The LabPaq referenced in this manual is produced by Hands-On Labs‚ Inc. which holds and reserves all copyrights on the intellectual properties associated with the LabPaq’s
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Math Practice Lab Pre-Lab Questions: 1. The rules concerning handling significant figures are as follows: When dividing/multiplying The answer has no more significant digits than the number with the fewest significant digits (the least precise figure). Round off after calculations have been performed. When adding/subtracting Answer has no more places than the addend‚ minuend‚ or subtrahend with the fewest number of decimal places. Significant figures are irrelevant when adding/subtracting
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basic. Any number lower is acidic. In this lab we have tested a number of substances on the pH scale and their effects when being poured on different colors of litmus paper. You can neutralize a base and an acid by combining the right amount between them. In the term H3O+ + OH- --> 2H2O you can see it neutralized. Just like in math if you add a negative to a positive they can neutralize each other out if it’s the same number or amount on both sides. In this lab we saw the effects of putting these substances
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Treatment of Results 1) What is the role of 0.25M sucrose as the medium for the fractionation process? Cold sucrose does not chemically react with cell organelles Due to the density and size of sucrose molecules‚ it is able to suspend pellets for configuration while providing a solution where the centrifugation can be better balanced Sucrose offers a liquid medium in which less dense fractions can be poured off as supernatant at the end of each centrifugation step. 0.25M sucrose solution
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dark reactions affect the rate of photosynthesis in chloroplasts. We will also be using a reference solution made of water‚ phosphate buffer‚ and active chloroplasts. The purpose of this solution will be used to set the transmittance level for the experiment. The control solution‚ which is different than the reference solution‚ is comprised of water‚ phosphate buffer‚ and DPIP. It will be
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Lawrence Joy Pengda Jin Jing Shang Hanish Mandru 1. What are the various types of malware? How do worms differ from viruses? Do Trojan horses carry viruses or worms? a. The various types of malware are viruses‚ worms‚ Trojan horses‚ polymorphic threats‚ virus and worm hoaxes‚ back door or trap door‚ denialofservice and distributed denialofservice‚ and mail bomb. b. A virus must be executed such as opening an infected email attachment while a worm can be initiated with or without the user downloading or executing the
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