cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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Introduction Chemical kinetics is the study of reaction rates. A reaction rate is the speed of the change in either reactants or products over a period of time. General kinetic rate equation is: Where [A] and [B] are the concentration of the species in the reaction. The variable k is the rate constant‚ which is a function of time and catalyst presence. The variables m and n are the order of reaction for their respective species concentration. The higher the value of the reaction order the
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In this lab we are going to be observing the decomposition of piglets over a month’s time. There are theory questions that have been given to us before and after the lab. We look back at our original theory to see where we went wrong‚ and then correct it. The lab was disgusting‚ surprising‚ and very interesting. The first questioned to be answered is which piglet decomposes faster‚ a piglet that is in its natural state‚ that is burnt‚ that is buried‚ and that is buried in a wooden box? With
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The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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Biochemistry is the chemistry of biological systems. The practical component of biochemistry is aimed at developing your interest in and understanding modern biochemical and molecular biological experimentation. The techniques learnt in the biochemistry lab will be applicable to all life sciences. THE OBJECTIVES OF THE BIOCHEMISTRY LABORATORY INCLUDE: (1) Learning the theory behind the techniques and biochemical pathways (2) Learning the physical skills and techniques of modern experimental biochemistry
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Abstract: The Enzyme Lab results where when the liver was frozen‚ its reaction was fast‚ and when it was hot‚ it was slow‚ and the liver that was at room temperature reacted slowly to medium. Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature‚ pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions‚ which would otherwise
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Littlefield Labs 1 Capacity Management at Littlefield Labs I. Introduction There are 3 stations in the game called sample preparing‚ testing‚ and centrifuging‚ while there are 4 steps to process the jobs. Before the game started‚ we tried to familiarize with the process of the laboratories and calculating the costs (both fixed and variable costs) based on the information on the sheet given. We did not intend to buy any machines too early‚ as we wanted to see the demand fluctuation and the
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components are altered. In this lab experiment‚ we will be doing an in-depth research of exactly what happens to the enzymes‚ when it happens‚ and why it denatures the way it does. b. Hypothesis Materials and Methods a. Materials 50ml beaker of fresh potato catalase Reaction chamber Ring stand and clamp 10ml graduated cylinder 100ml graduated cylinder 3% hydrogen peroxide Pan (water bath) Pipette Hot plate Ice Thermometer Boiled Catalase Buffers of varying pH: 4‚7‚10 Distilled
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Since the Grignard reagent can easily react with water‚ all glassware including the 25 ml round bottom flask‚ magnetic stir bar‚ 3 and 5 ml conical vial‚ 50 mL Erlenmeyer flask‚ claisen adapter‚ drying tube and 5 glass pasteur pipets were first added to a 250mL beaker and placed in the oven for 30 minutes. After the completion of the thirty minutes‚ 0.150 g of shiny magnesium turnings and a stir bar was first added to the round bottom flask and the claisen adapter along with the drying tube packed
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