INSTRUCTOR’S RESOURCE MANUAL CHAPTER ELEVEN Critical Chain Project Scheduling To Accompany PROJECT MANAGEMENT: Achieving Competitive Advantage By Jeffrey K. Pinto CHAPTER 11 PROJECT PROFILE – Canada’s Oil Sands Recovery Projects INTRODUCTION 11.1 THE THEORY OF CONSTRAINTS AND CRITICAL CHAIN PROJECT SCHEDULING Theory of Constraints Common Cause and Special Cause Variation 11.2 CCPM AND THE CAUSES OF PROJECT DELAY Method One: Overestimation of Individual Activity Durations
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50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded and the column was washed twice (i.e. centrifuged); first in 30 mL of low salt Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl)
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further analysis on the protein to be conducted‚ such as its concentration (Bradford assay)‚ its molecular weight‚ and its biological activity. 2. Overview of experiments Buffer Preparation Add the liquid sodium phosphate‚ solid sodium chloride and liquid imidazole in proper amount and concentration for three different buffers. Save 100µL of each cell lysate into two tubes for lab 3 and 4. DHFR Protein Purification Keep every reagent on ice. Label all 12 collection tubes. Remove the
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brought into room temperature. In 2ml eppendrof tube approximately 220mg stool sample were taken. Washing buffer was prepared by adding distilled water . Procedure for Purification of DNA from Stool sample: i. 220mg stool sample were collected in a 2ml tubes and placed it on ice. ii. Added 2ml Buffer ASL to each stool tube. Used pipet to wash the stool sample from the spoon while transferring the buffer. Vortexed continuously for 1minute or until the stool samples was thoroughly homogenized. iii. Pipet
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Prior to use‚ the column was equilibrated using 20mM sodium phosphate buffer (pH6.5). The column was washed for 10 CV with the same buffer after the protein loading and the bound proteins were eluted with a linear gradient of NaCl (0-0.5M) in the same buffer over 10 CV at a flow rate of 1mL/min. The negative ions (Cl-) in the salt solution were competed with protein in binding to the resin. Fractions of 2mL were
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that a certain full-color (24-bit per pixel) RGB raster system has a 512-by-512 frame buffer‚ how many distinct color choices (intensity levels) would we have available? How many different colors could we display at any one time? Total number of distinct color available is 224 Total number of colors we could display at one time is 512 x 512 2 Assuming that a certain RGB raster system has 512*512 frame buffer with 12 bit per pixel and color lookup table with 24 bit for each entry 1 How many
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relationship between the capital base of banks and the 2007-2010 global financial crisis. Using your own research‚ cite at least two examples of real world financial institutions. 2. Why is there a perceived need of counter-cyclical buffers. Define and discuss how counter-cyclical buffers might best be structured. Cite at least two peer-reviewed journal articles in providing your arguments. 3. Discuss the need to include the leverage ratio and off-balance sheet assets in Basel III. 4. What measures should
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Abstract This experiment followed an exact protocol from EDVOTEK® Kit #17. This kit was designed to use western blot analysis to detect proteins from Non-fat powdered milk. The experiment had to be run two times before the expected outcome was reached. However‚ after the second attempt‚ it was successful. The gel ran well‚ and the proteins from the gel were successfully transferred to the membrane provided in the kit. Introduction The name western blot was given to the technique by W. Neal
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CaCl2*2H2O)(1mol Na2CO3/1mol CaCl2*2H2O)(106g Na2CO3/1mol Na2CO3) = 0.72g Na2CO3. The final‚ when its dried in the paper filtered weighed at 1.6 grams. Experiment and Observations: As I performed the lab‚ I had to retrieve all my equipment’s from my labpaq. As I started to setup‚ I had to weigh out 1.0 gram of CaCl2*2H2O on the digital scale. After it was weighed‚ it was added to the 100 mL beaker and 25 mL of distilled was added and stirred. Next‚ I had to figure out the amount of the second reactant
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of the cell‚ and that is an extraction buffer would be needed to acquire the DNA. This experiment had no control group‚ however‚ if there was a control group it might be a group with no extraction buffer. The independent variable in this experiment is the extraction buffer. The dependent variable in this experiment is the ability to extract DNA. The constants in this experiment include‚ the shape and size of the coffee filter‚ the amount of extraction buffer used‚ and the amount of isopropyl alcohol
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